Abstract
BASAL transcription by human RNA polymerase II requires the coordinate action of several ancillary factors (TFIIA-J)1 and can be regulated by various promoter-specific DNA binding proteins. An additional class of factors, called coactivators, are dispensable for basal transcription but are indispensable for regulation by transcriptional activators2–4. Biochemical studies established that some coactivators are associated with the TATA-binding protein (TBP) to form the TFIID complex3–6. We therefore set out to define the relationship between TBP and these TBP-associated factors (TAFs). Here we describe the cloning, expression and properties of the first human TAF, hTAFII250. The hTAFII250 gene is identical to a gene, CCG1 (refs 7, 8), implicated in cell-cycle progression. Recombinant hTAFII250 binds directly to TBP both in vitro and in yeast, and participates in the formation of the TFIID complex. This largest TAF may therefore play a central role in TFIID assembly by interacting with both TBP and other TAFs, as well as serving to link the control of transcription to the cell cycle.
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References
Zawel, L. & Reinberg, D. Prog. Nucl. Acids Res. molec. Biol. 44, 67–108 (1993).
Pugh, B. F. & Tjian, R. Cell 61, 1187–1197 (1990).
Dyniacht, B. D., Hoey, T. & Tjian, R. Cell 55, 563–576 (1991).
Tanese, N., Pugh, B. F. & Tjian, R. Genes Dev. 5, 2212–2224 (1991).
Pugh, B. F. & Tjian, R. Genes Dev. 5, 1935–1945 (1991).
Hoey, T. et al. Cell 72, 1–20 (1993).
Sekiguchi, T., Miyata, T. & Nishimoto, T. EMBO J. 7, 1683–1687 (1988).
Sekiguchi, T., Nohiro, Y., Nakamura, Y., Hisamoto, N. & Nishimoto, T. Molec. cell. Biol. 11, 3317–3325 (1991).
Talavera, A. & Basilico, C. J. cell. Physiol. 92, 425–436 (1977).
Fields, S. & Song, O. Nature 340, 245–246 (1989).
Nikolov, D. B. et al. Nature 360, 40–46 (1992).
Liu, H. T. et al. J. biol. Chem. 260, 3269–3274 (1985).
Zhou, Q., Liebermann, P. M., Boyer, T. G. & Berk, A.-J. Genes Dev. 6, 1964–1974 (1992).
Kaelin, W. G. et al. Cell 70, 351–364 (1992).
Jantzen, H.-M., Chow, A. M., King, D. S. & Tjian, R. Genes Dev. 6, 1950–1963 (1992).
Harlow, E. & Lane, D. Antibodies. A Laboratory Manual (Cold Spring Harbor Laboratory Press, New York, 1988).
Ruppert, S., Cole, T. J., Boshart, M., Schmid, E. & Schuetz, G. EMBO J. 11, 1503–1512 (1992).
Short, J. M., Fernandez, J. M., Sorge, J. A. & Huse, W. D. Nucleic Acids Res. 16, 7583–7600 (1988).
Field, J. et al. Molec. cell. Biol. 8, 2159–2165 (1988).
Peterson, M. G., Tanese, N., Pugh, B. F. & Tjian, R. Science 248, 1625–1630 (1990).
Dynlacht, B. D., Attardi, L. D., Admon, A., Freeman, M. & Tjian, R. Genes Dev. 3, 1677–1688 (1989).
Chien, C.-T., Bartel, P. L., Sternglanz, R. & Fields, S. Proc. natn. Acad. Sci. U.S.A. 88, 9578–9582 (1991).
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Ruppert, S., Wang, E. & Tjian, R. Cloning and expression of human TAFII250: a TBP-associated factor implicated in cell-cycle regulation. Nature 362, 175–179 (1993). https://doi.org/10.1038/362175a0
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DOI: https://doi.org/10.1038/362175a0
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