Abstract
ANTIGENSpresented to CD4+T cells derive primarily from exogenous proteins that are processed into peptides capable of binding to class II major histocompatibility complex (MHC) molecules in an endocytic compartment1–4. In contrast, antigens presented to CD8+T cells derive mostly from proteins processed in the cytosol, and peptide loading onto class I MHC molecules in an early exocytic compartment is dependent on a transporter for antigen presentation encoded in the class II MHC region5–11. Endogenous cytosolic antigen can also be presented by class II molecules12,13. Here we show that, unlike class I-restricted recognition of antigen, HLA-DR 1-restricted recognition of cytosolic antigen occurs in mutant cells without a transporter for antigen presentation. In contrast, DR1-restricted recognition of a short cytosolic peptide is dependent on such a transporter. Thus helper T-cell epitopes can be generated from cytosolic antigens by several mechanisms, one of which is distinct from the classical class I pathway.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Unanue, E. R. & Allen, P. M. Science 236, 551–557 (1987).
Morrison, L. A., Lukacher, A. E., Braciale, V. L., Fan, D. P. & Braciale, T. J. J. exp. Med. 163, 903–921 (1986).
Rudensky, A. Y., Preston-Hurlburt, P., Hong, S.-C., Barlow, A. & Janeway, C. A. Jr Nature 353, 622–627 (1991).
Germain, R. N. & Hendrix, L. R. Nature 353, 134–139 (1991).
Townsend, A. & Bodmer, H. A. Rev. Immun. 7, 601–624 (1989).
Cox, J. H., Bennink, J. R. & Yewdell, J. W. J. exp. Med. 174, 1629–1637 (1991).
Monaco, J. J., Cho, S. & Attaya, M. Science 250, 1723–1726 (1990).
Deverson, E. V. et al. Nature 348, 738–741 (1990).
Trowsdale, J. et al. Nature 348, 741–744 (1990).
Spies, T. et al. Nature 348, 744–747 (1990).
Spies, T. et al. Nature 355, 644–646 (1992).
Nuchtern, J. G., Biddison, W. E. & Klausner, R. D. Nature 343, 74–76 (1990).
Jaraquemada, D., Marti, M. & Long, E. O. J. exp. Med. 172, 947–954 (1990).
Townsend, A. R. M., Bastin, J., Gould, K. & Brownlee, G. G. Nature 324, 575–577 (1986).
Whitton, J. L. & Oldstone, M. B. A. J. exp. Med. 170, 1033–1038 (1989).
Gould, K., Cossins, J., Bastin, J., Brownlee, G. G. & Townsend, A. J. exp. Med. 170, 1051–1056 (1989).
Sweetser, M. T., Morrison, L. A., Braciale, V. L. & Braciale, T. J. Nature 342, 180–182 (1989).
DeMars, R., Chang, C. C., Shaw, S., Reitnauer, P. J. & Sondel, P. M. Hum. Immun. 11, 77–97 (1984).
Spies, T. & DeMars, R. Nature 351, 323–324 (1991).
Ceman-Bogner, S., Rudersdorf, R., Long, E. O. & DeMars, R. J. Immun. (in the press).
Long, E. O. New Bio. 4, 274–282 (1992).
Kavathas, P., Bach, F. H. & DeMars, R. Proc. natn. Acad. Sci. U.S.A. 77, 4251–4255 (1980).
Gould, K. G., Scotney, H., Townsend, A. R. M., Bastin, J. & Brownlee, G. G. J. exp. Med. 166, 693–701 (1987).
Earl, P. L., Koenig, S. & Moss, B. J. Virol. 65, 31–41 (1991).
Chakrabarti, S., Brechling, K. & Moss, B. Molec. cell. Biol. 5, 3403–3409 (1985).
Karp, D. R. & Long, E. O. J. exp. Med. 175, 415–424 (1992).
Smith, G. L., Levin, J. Z., Palese, P. & Moss, B. Virology 160, 336–345 (1987).
Kozak, M. Nucleic Acids Res. 15, 8125–8128 (1987).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Malnati, M., Marti, M., LaVaute, T. et al. Processing pathways for presentation of cytosolic antigen to MHC class II-restricted T cells. Nature 357, 702–704 (1992). https://doi.org/10.1038/357702a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/357702a0
This article is cited by
-
Introducing Molecular Chaperones into the Causality and Prospective Management of Autoimmune Hepatitis
Digestive Diseases and Sciences (2023)
-
The CCR4-NOT complex contributes to repression of Major Histocompatibility Complex class II transcription
Scientific Reports (2017)
-
Beyond the classical: Influenza virus and the elucidation of alternative MHC class II-restricted antigen processing pathways
Immunologic Research (2011)
-
Variation in the ATP-binding cassette transporter 2 gene is a separate risk factor for systemic lupus erythematosus within the MHC
Genes & Immunity (2009)
-
Autophagy in CD4+ T-cell immunity and tolerance
Cell Death & Differentiation (2009)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.