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Homing of a DNA endonuclease gene by meiotic gene conversion in Saccharomyces cerevisiae

Nature volume 357, pages 301–306 (1992)Cite this article

Abstract

An unusual protein splicing reaction joins the N-terminal segment (A) and the C-terminal segment (C) of the 119K primary translation product (ABC) of the yeast VMA1 gene to yield a 69K vacuolar H+-ATPase subunit (AC) and an internal 50K polypeptide (B). This 50K protein is a site-specific DNA endonuclease that shares 34% identity with the homothallic switching endonuclease. The site cleaved by the VMA1-derived endonuclease exists in a VMA1 allele that lacks the derived endonuclease segment of the open reading frame. Cleavage at this site only occurs during meiosis and initiates 'homing', a genetic event that converts a VMA1 allele lacking the endonuclease coding sequence into one that contains it.

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  1. Division of Biochemistry and Molecular Biology, Department of Molecular and Cell Biology, Room 401 Barker Hall, University of California, Berkeley, California, 94720, USA

    Frederick S. Gimble & Jeremy Thorner

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  2. Jeremy Thorner
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Gimble, F., Thorner, J. Homing of a DNA endonuclease gene by meiotic gene conversion in Saccharomyces cerevisiae. Nature 357, 301–306 (1992). https://doi.org/10.1038/357301a0

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