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Cloning and domain structure of the mammalian splicing factor U2AF

Nature volume 355, pages 609–614 (1992)Cite this article

Abstract

A complementary DNA clone encoding the large subunit of the essential mammalian premessenger RNA splicing component U2 snRNP auxiliary factor (U2AF65) has been isolated and expressed in vitro. It contains two functional domains: a sequence-specific RNA-binding region composed of three ribonucleo protein-consensus sequence domains, and an arginine/serine-rich motif necessary for splicing but not for binding to pre-mRNA.

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Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts, 02138, USA

    Phillip D. Zamore

  2. Laboratory of Molecular and Cellular Physiology, Harvard Medical School, Boston, Massachusetts, 02115, USA

    James G. Patton

  3. Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester, Massachusetts, 01605, USA

    Michael R. Green

Authors
  1. Phillip D. Zamore
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  2. James G. Patton
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  3. Michael R. Green
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Zamore, P., Patton, J. & Green, M. Cloning and domain structure of the mammalian splicing factor U2AF. Nature 355, 609–614 (1992). https://doi.org/10.1038/355609a0

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  • DOI: https://doi.org/10.1038/355609a0

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