Abstract
THE transcription factor NF-κB is a heterodimer consisting of two proteins encoded by different members of the rel gene family (p50 and p65)1–7. The p50 subunit is unusual among DNA-binding proteins in that its functional form is encoded in an open reading frame of relative molecular mass 105,000 (p105; ref. 4). The N-terminal region of this open reading frame encodes p50, whereas the remaining C terminus contains ankyrin repeats. Although p50 binds to DNA, full-length p105 translated in vitro does not4,5. The mechanism by which p50 is generated in vivo, and the fate of the C-terminal region of p105 have not been established. Here we show that functional p50 is produced by ATP-dependent pro-teolysis of p105. Moreover, we find that the C-terminal half of p105 is not required for processing in vivo, and is rapidly degraded on processing. We propose that the C-terminal region of p105 is involved in the cytoplasmic assembly of the complex between the p50/p65 heterodimer and the inhibitor IkB.
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References
Kawakami, K., Scheidereit, C. & Roeder, R. Proc. natn. Acad. Sci. U.S.A. 85, 4700–4704 (1988).
Baeuerle, P. A. & Baltimore, D. Genes Dev. 3, 1689–1699 (1989).
Lenardo, M. J. & Baltimore, D. Cell 58, 227–229 (1989).
Kieran, M. et al. Cell 62, 1007–1018 (1990).
Ghosh, S. et al. Cell 62, 1019–1029 (1990).
Nolan, G. et al. Cell 64, 961–969 (1991).
Ruben, S. M. et al. Science 251, 1490–1492 (1991).
Riviere, Y. et al. Nature 350, 625–626 (1991).
Munro, S. & Pelham, H. R. B. Cell 48, 899–907 (1987).
Field, J. et al. Molec. cell. Biol. 8, 2159–2165 (1988).
Lenardo, M. J., Fan, C.-M., Maniatis, T. & Baltimore, D. Cell 57, 287–294 (1989).
Fan, C.-M. & Maniatis, T. EMBO J. 8, 101–110 (1989).
Hershko, A. J. biol. Chem. 263, 15237–15240 (1988).
Rechsteiner, M. Cell 66, 615–618 (1991).
Haskill, S. et al. Cell 65, 1281–1289 (1991).
Ballard, D. W. et al. Cell 63, 803–814 (1990).
Inoue, J. et al. Proc. natn. Acad. Sci. U.S.A. 88, 3715–3719 (1991).
Tanaka, M. & Herr, W. Cell 60, 375–386 (1990).
Harlow, E. & Lane, D. Antibodies, A Laboratory Manual (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1988).
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Fan, CM., Maniatis, T. Generation of p50 subunit of NF-kB by processing of p105 through an ATP-dependent pathway. Nature 354, 395–398 (1991). https://doi.org/10.1038/354395a0
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DOI: https://doi.org/10.1038/354395a0
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