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Homology of a yeast actin-binding protein to signal transduction proteins and myosin-I

Nature volume 343, pages 288–290 (1990)Cite this article

Abstract

IN yeast, the cortical actin cytoskeleton seems to specify sites of growth of the cell surface1,2. Because the actin-binding protein ABPlp is associated with the cortical cytoskeleton of Saccharomyces cerevisiae, it might be involved in the spatial organization of cell surface growth3. ABPlp is localized to the cortical cytoskeleton and its overproduction causes assembly of the cortical actin cytoskeleton at inappropriate sites on the cell surface, resulting in delocalized surface growth. We have now cloned and sequen-ced the gene encoding ABPlp. ABPlp is a novel protein with a 50 amino-acid C-terminal domain that is very similar to the SH3 domain in the non-catalytic region of nonreceptor tyrosine kinases (including those encoded by the proto-oncogenes c-src and c-abl), in phopholipase Cγ and in α-spectrin. We also identified an SH3-related motif in the actin-binding tail domain of myosin-I. The identification of SH3 domains in a family of otherwise unrelated proteins that associate with the membrane cytoskeleton indicates that this domain might serve to bring together signal transduction proteins and their targets or regulators, or both, in the membrane cytoskeleton.

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Author notes

  1. David G. Drubin

    Present address: Department of Molecular and Cell Biology, 471 Life Sciences Addition, University of California, Berkeley, California, 94720

  2. Jon Mulholland & David Botstein

    Present address: Genentech Inc., 460 Point San Bruno Boulevard, South San Francisco, California, 94080, USA

  3. Zhimin Zhu

    Present address: Department of Biochemistry and Pharmacology, Harvard Medical School, Boston, Massachusetts, 02115, USA

Authors and Affiliations

  1. Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA

    David G. Drubin, Jon Mulholland, Zhimin Zhu & David Botstein

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  1. David G. Drubin
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  4. David Botstein
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Drubin, D., Mulholland, J., Zhu, Z. et al. Homology of a yeast actin-binding protein to signal transduction proteins and myosin-I. Nature 343, 288–290 (1990). https://doi.org/10.1038/343288a0

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