Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Association of a Ras-related protein with cytochrome b of human neutrophils

Nature volume 342, pages 198–200 (1989)Cite this article

Abstract

ACTIVATION of the superoxide generating system in human neutrophils is thought to involve the interaction or assembly of cytochrome b with other cytosolic and membrane proteins. We have now co-isolated by conventional purification procedures a protein of relative molecular mass 22,000 with cytochrome b. This Ras-related protein is not a fragment of either of the subunits of cytochrome b, and its primary structure, as determined by the sequencing of its complementary DNA, is identical to that predicted from a recently cloned ras- related gene, rap 1 (also termed Krev-1). Immunoaffinity purification on anti-cytochrome and anti-Ras immunoaffinity matrices indicates an association between cytochrome b and the Ras-related protein. The association of a Ras-related GTP-binding protein with cytochrome b of human neutrophils could indicate a role for such a protein in the transduction, regulation or structure of the superoxide generating system.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Hematotogy/Oncology Clinics of North America Vol. 2, Nos 1 and 2 (ed. Curnutte, J. T.) (Saunders, Philadelphia, 1988).

  2. Babior, B. M., Kipnes, R. S. & Curnutte, J. T. J. clin. Invest. 52, 741–744 (1973).

    Article  CAS  Google Scholar 

  3. Karnovsky, M. L. & Badwey, J. A. J. clin. chem. Biochem. 21, 545–553 (1983).

    CAS  Google Scholar 

  4. Babior, B. M. & Kipnes, R. S. Blood 50, 517–524 (1977).

    CAS  PubMed  Google Scholar 

  5. Segal, A. W. & Jones, O. T. G. Nature 276, 515–517 (1978).

    Article  ADS  CAS  Google Scholar 

  6. Segal, A. W., Jones, O. T. G., Webster, D. & Allison, A. C. Lancet ii, 446–449 (1978).

    Article  Google Scholar 

  7. Dinauer, M. C., Orkin, S. H., Brown, R., Jesaitis, A. J. & Parkos, C. A. Nature 327, 717–720 (1987).

    Article  ADS  CAS  Google Scholar 

  8. Curnutte, J. T. & Babior, B. M. in Advances in Human Genetics (eds Harris, H. & Hirschorn, K.) 229–297 (Plenum, New York, 1987).

    Book  Google Scholar 

  9. McPhail, L. C., Shirley, P. S., Clayton, C. C. & Snyderman, R. J. clin. Invest. 75, 1735–1739 (1985).

    Article  CAS  Google Scholar 

  10. Curnutte, J. T. J. clin. Invest. 75, 1740–1743 (1985).

    Article  CAS  Google Scholar 

  11. Volpp, B. D., Nauseef, W. M. & Clark, R. A. Science 242, 1295–1297 (1988).

    Article  ADS  CAS  Google Scholar 

  12. Hiroyuki, N., Rotrosen, D., Gallin, J. I. & Malech, H. L. Science 242, 1298–1301 (1988).

    Article  ADS  Google Scholar 

  13. Pick, E., Bromberg, Y., Shpagen, S. & Gadba, R. J. biol. Chem. 262, 16476–16483 (1987).

    CAS  PubMed  Google Scholar 

  14. Segal, A. W., Heyworth, P. G., Cockcroft, S. & Barrowman, M. M. Nature 316, 547–549 (1985).

    Article  ADS  CAS  Google Scholar 

  15. Hayakawa, T., Suzuki, K., Suzuki, S., Andrews, P. C. & Babior, B. M. J. biol. Chem. 261, 9109–9115 (1986).

    CAS  PubMed  Google Scholar 

  16. Umei, T., Takeshige, K. & Minakami, S. J. biol. Chem. 261, 5222–5232 (1986).

    Google Scholar 

  17. Light, D. R., Walsh, C., O'Callaghan, A. M., Goetzl, E. J. & Tauber, A. I. Biochemistry 20, 1468–1476 (1984).

    Article  Google Scholar 

  18. Borregaard, N. & Tauber, A. I. J. bio., Chem. 259, 47–52 (1984).

    CAS  Google Scholar 

  19. Kakinuma, K., Fukuhara, Y. & Kaneda, M. J. biol. Chem. 262, 12316–12322 (1987).

    CAS  PubMed  Google Scholar 

  20. Parkinson, J. F. & Gabig, T. G. J. biol. Chem. 263, 8859–8863 (1988).

    CAS  PubMed  Google Scholar 

  21. Gabig, T. G., English, D., Akard, L. P. & Schell, M. J. J. biol. Chem. 262, 1685–1690 (1987).

    CAS  PubMed  Google Scholar 

  22. Parkos, C. A., Allen, R. A., Cochrane, C. G. & Jesaitis, A. J. J. clin. Invest. 80, 732–742 (1987).

    Article  CAS  Google Scholar 

  23. Parkos, C. A. et al. Proc. natn. Acad. Sci. U.S.A. 85, 3319–3323 (1988).

    Article  ADS  CAS  Google Scholar 

  24. Chesa, P. G., Rettig, W. J., Melamed, M. R., Old, L. J. & Niman, H. L. Proc. natn. Adad. Sci. U.S.A. 84, 3234–3238 (1987).

    Article  ADS  CAS  Google Scholar 

  25. Royer-Pokora, B. et al. Nature 322, 32–38 (1986).

    Article  ADS  CAS  Google Scholar 

  26. Pizon, V., Chardin, P., Lerosey, I., Olofsson, B. & Tavitian, A. Oncogene 3, 201–204 (1988).

    CAS  PubMed  Google Scholar 

  27. Kitayama, H., Sugimoto, Y., Matsuzaki, T., Ikawa, Y. & Noda, M. Cell 56, 77–84 (1989).

    Article  CAS  Google Scholar 

  28. Wray, W., Boulikas, T., Wray, V. & Hancock, R. J. analyt. Biochem. 118, 197–203 (1981).

    Article  CAS  Google Scholar 

  29. Parkos, C. A., Cochrane, C. G., Schmitt, M. & Jesaitis, A. J. J. biol. Chem. 260, 6541–6547 (1985).

    CAS  PubMed  Google Scholar 

  30. Lomax, K. J., Leto, T. L., Nunoi, H., Gallin, J. I. & Malech, H. L. Science 245, 409–412 (1989).

    Article  ADS  CAS  Google Scholar 

  31. Trahey, M. McCormick, F. Science 238, 542–545 (1987).

    Article  ADS  CAS  Google Scholar 

  32. Barbacid, M. A. Rev. Biochem. 56, 779–827 (1987).

    Article  CAS  Google Scholar 

  33. Quinn, M. T., Parkos, C. A. & Jesaitis, A. J. Biochim. biophys. Acta (in the press).

  34. Chou, P. Y. & Fasman, G. D. Biochemistry 13, 222–245 (1974).

    Article  CAS  Google Scholar 

  35. Doolittle, R. F. Of URFS and ORFS, 63–92 (University Science Books, Mill Valley, California, 1986).

    Google Scholar 

  36. Schneider, C., Newman, R. A., Sutherland, D. R., Asser, U. & Greaves, M. F. J. biol. Chem. 257, 10766–10769 (1982).

    CAS  PubMed  Google Scholar 

Download references

Author information

Author notes

  1. Charles A. Parkos

    Present address: Department of Pathology, Brigham & Women's Hospital, 75 Francis Street, Boston, Massachusetts, 02115, USA

Authors and Affiliations

  1. Department of Chemistry, Montana State University, Bozeman, Montana, 59717, USA

    Mark T. Quinn & Algirdas J. Jesaitis

  2. Research Institute of Scripps Clinic, 10666 North Torrey Pines Road, La Jolla, California, 92037, USA

    Charles A. Parkos & Leslie Walker

  3. Division of Hematology-Oncology, Children's Hospital and Dana-Farber Cancer Institute, Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, 02115, USA

    Stuart H. Orkin & Mary C. Dinauer

  4. Howard Hughes Medical Institute, Children's Hospital, Boston, Massachusetts, 02115, USA

    Stuart H. Orkin

Authors
  1. Mark T. Quinn
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Charles A. Parkos
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Leslie Walker
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Stuart H. Orkin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mary C. Dinauer
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Algirdas J. Jesaitis
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Quinn, M., Parkos, C., Walker, L. et al. Association of a Ras-related protein with cytochrome b of human neutrophils. Nature 342, 198–200 (1989). https://doi.org/10.1038/342198a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/342198a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing