Abstract
CLASS I and class II MHC-restricted T lymphocytes recognize non-native forms of antigen1–7. The presentation of antigen to these two classes of T lymphocytes can occur through distinct pathways8–10. Several mechanisms, including differences in antigen processing in different intracellular compartments, have been proposed to account for these pathway differences9–14. Here we describe a T-cell epitope located on the influenza virus haemag-glutinin, which is recognized by both class I and class II MHC-restricted cytolytic T lymphocytes (CTL). When expressed de novo in target cells, from a synthetic minigene15 encoding only the epitope, this pre-processed antigenic site is recognized by class I but not class II MHC-restricted T lymphocytes, even though target cells treated with the exogenously introduced peptide can be recognized by both classes of T cells. Because endogenous expression of the pre-processed antigenic fragment results in differential presentation to class I and class II MHC-restricted CTL, differences between the two different pathways of presentation could lie not at the level of processing but at the level of targeting and/or interaction of processed antigen with MHC.
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References
Ziegler, H. K. & Unanue, E. R. Proc. natn. Acad. Sci. U.S.A. 79, 175–178 (1982).
Unanue, E. R. A. Rev. Immun. 4, 395–428 (1984).
Townsend, A. R. M., Gotch, F. M. & Davey, J. Cell 42, 457–467 (1985).
Townsend, A. R. M. et al., Cell 44, 959–968 (1986).
Wabuke-Bunoti, M., Fan, D. P. & Braciale, T. J. J. Immun. 127, 1122–1125 (1981).
Maryanski, J. L., Pala, P., Corradin, G., Jordan, B. R. & Cerrotini, J. C. Nature 324, 578–579 (1986).
Gotch, F., Rothbard, J., Howland, K., Townsend, A. & McMichael, A. Nature 326, 881–882 (1987).
Morrison, L. A., Lukacher, A. E., Braciale, V. L., Fan, D. P. & Braciale, T. J. J. exp. Med. 163, 903–921 (1986).
Braciale, T. J. et al. J. exp. Med. 166, 678–692 (1987).
Moore, M. W., Carbone, F. R. & Bevan, M. J. Cell 54, 777–785 (1988).
Germain, R. N. Nature 322, 687–689 (1986).
Germain, R. N. Cell 54, 441–444 (1988).
Jin, Y., Shih, J. W.-K. & Berkower, I. J. exp. Med. 168, 293–306 (1988).
Jacobson, S., Sekaly, R. P., Jacobson, C. L., McFarland, H. F. & Long, E. O. J. Virol. 63, 1756–1762 (1989).
Sweetser, M. T., Braciale, V. L. & Braciale, T. J. J. Immun. 141, 3324–3328 (1988).
Braciale, T. J., Sweetser, M. T., Morrison, L. A., Kittlesen, D. J. & Braciale, V. L. Proc. natn. Acad. Sci. U.S.A. 86, 277–281 (1989).
Lukacher, A. E., Morrison, L. A., Braciale, V. L., Malissen, B. & Braciale, T. J. J. exp. Med. 162, 171–187 (1985).
Bennink, J. R., Yewdell, J. W., Smith, G. L., Moller, C. & Moss, B. Nature 311, 578–579 (1984).
Coupar, B. E. H., Andrew, M. E., Both, G. W. & Boyle, D. B. Eur. J. Immun. 16, 1479–1487 (1986).
Townsend, A. R. M., Bastin, J., Gould, K. & Brownlee, G. G. Nature 324, 575–577 (1986).
Gething, M.-J., McCammon, K. & Sambrook, J. Cell 46, 939–950 (1986).
Doyle, C., Roth, M. G., Sambrook, J. & Gething, M.-J. J. Cell Biol. 100, 704–714 (1985).
Lazarovits, J. & Roth, M. Cell 53, 743–752 (1988).
Nuchtern, J., Bonifacino, J., Biddison, W. & Klausner, R. Nature 339, 223–226 (1989).
Yewdell, J. W. & Bennink, J. R. Science 244, 1072–1075 (1989).
Blum, J. S. & Cresswell, P. Proc. natn. Acad. Sci. U.S.A. 85, 3975–3979 (1988).
Chakrabarti, S., Brechling, K. & Moss, B. Molec. cell. Biol. 5, 910–923 (1981).
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Sweetser, M., Morrison, L., Braciale, V. et al. Recognition of pre-processed endogenous antigen by class I but not class II MHC-restricted T cells. Nature 342, 180–182 (1989). https://doi.org/10.1038/342180a0
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DOI: https://doi.org/10.1038/342180a0
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