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The codon CUG is read as serine in an asporogenic yeast Candida cylindracea

Nature volume 341, pages 164–166 (1989)Cite this article

Abstract

DEVIATIONS from the universal genetic code have been reported for several microorganisms. Termination codons are used for cod-ing some amino acids in Paramecium1,2, Mycoplasma3 or Tetrahymena4,5, and in Escherichia coli, the UGA termination codon is used to code for selenocysteine6. In mitochondria, the changes of sense codons to termination codons or to codons encoding other amino acids have also been reported7,8. Here we report another example of divergence from the universal code, this time in a non-spore-forming yeast Candida cylindracea, in which the uni-versal codon for leucine, CUG, is used to code for serine. This conclusion is based on the observations that: (1) the amino-acid composition and the partial amino-acid sequences of an extracel-lular lipase from this yeast agreed with those deduced from the complementary DNA if CUG was assumed to specify serine; and (2) serine, but not leucine, was incorporated into a polypeptide in a cell-free translation system from this yeast in the presence of a synthetic CUG oligomer.

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References

  1. Caron, F. & Meyer, E. Nature 314, 185–188 (1985).

    Article  ADS  CAS  Google Scholar 

  2. Preer, J. R. Jr, Preer, L. B., Rudman, B. M. & Barnett, A. J. Nature 314, 188–190 (1985).

    Article  ADS  CAS  Google Scholar 

  3. Yamao, F. et al. Proc. natn. Acad Sci. U.S.A. 82, 2306–2309 (1985).

    Article  ADS  CAS  Google Scholar 

  4. Horowitz, S. & Gorovsky, M. A. Proc. natn Acad. Sci. U.S.A. 82, 2452–2455 (1985).

    Article  ADS  CAS  Google Scholar 

  5. Kuchino, Y., Hanyu, N., Tashiro, F. & Nishimura, S. Proc. natn. Acad. Sci. U.S.A. 82, 4758–4762 (1985).

    Article  ADS  CAS  Google Scholar 

  6. Leinfelder, W., Zehelein, E., Mandrand-Berthelot, M.-A. & Böck, A. Nature 331, 723–725 (1988).

    Article  ADS  CAS  Google Scholar 

  7. Bonitz, S. G. et al. Proc. natn. Acad Sci. U.S.A. 77, 3167–3170 (1980).

    Article  ADS  CAS  Google Scholar 

  8. Anderson, S. et al. Nature 290, 457–465 (1981).

    Article  ADS  CAS  Google Scholar 

  9. Yamada, K. & Machida, H. Nippon Nōgeikagaku kaishi 36, 858–860 (1962).

    Article  CAS  Google Scholar 

  10. Tomizuka, N., Ota, Y. & Yamada, K. Agric. Biol. Chem. 30, 576–584 (1966).

    CAS  Google Scholar 

  11. Normanly, J., Ogden, R. C., Horvath, S. J. & Abelson, J. Nature 321, 213–219 (1986).

    Article  ADS  CAS  Google Scholar 

  12. Inokuchi, H., Hoben, P., Yamao, F., Ozeki, H. & Söll, D. Proc. natn. Acad. Sci. U.S.A. 81, 5076–5080 (1984).

    Article  ADS  CAS  Google Scholar 

  13. Gorini, L. in Ribosomes, 791–803 (Cold Spring Harbor Laboratory, New York, 1974).

    Google Scholar 

  14. Yarus, M. & Thompson, R. in Gene Function in Prokaryotes (eds Beckwith, J., Davies, J. & Gallant, J. A.) 23–63 (Cold Spring Harbor Laboratory, New York, 1983).

    Google Scholar 

  15. Osawa, S. & Jukes, T. H. Trends Genet. 4, 191–198 (1988).

    Article  CAS  Google Scholar 

  16. Maniatis, T., Fritsch, E. F. & Sambrook, J. in Molecular Cloning: A Laboratory Manual 196 (Cold Spring Harbor Laboratory, New York, 1982).

    Google Scholar 

  17. Young, R. A. & Davis, R. W. Proc. natn. Acad Sci. USA. 80, 1194–1198 (1983).

    Article  ADS  CAS  Google Scholar 

  18. Young, R. A. & Davis, R. W. Science 222, 778–782 (1983).

    Article  ADS  CAS  Google Scholar 

  19. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  20. Melton, D. A. et al. Nucleic Acids Res. 12, 7035–7056 (1984).

    Article  CAS  Google Scholar 

  21. Krieg, P. A. & Melton, D. A. Nucleic Acids Res. 12, 7057–7070 (1984).

    Article  CAS  Google Scholar 

  22. Gasior, E., Herrera, F., Sadnik, I., McLaughlin, C. S. & Moldave, K. J. biol. Chem. 254, 3965–3969 (1979).

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Tokyo Research Laboratory, Meito Sangyo Co. Ltd., 2973-2 Ishikawacho, Hachioji, Tokyo, 192, Japan

    Yoshiyuki Kawaguchi, Hiroshi Honda, Junko Taniguchi-Morimura & Shinjiro Iwasaki

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  1. Yoshiyuki Kawaguchi
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  2. Hiroshi Honda
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  3. Junko Taniguchi-Morimura
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  4. Shinjiro Iwasaki
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Kawaguchi, Y., Honda, H., Taniguchi-Morimura, J. et al. The codon CUG is read as serine in an asporogenic yeast Candida cylindracea. Nature 341, 164–166 (1989). https://doi.org/10.1038/341164a0

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