Abstract
Two proteins of unknown function are encoded by 3' in-frame extensions of ubiquitin genes1. The polypeptides are synthesized as an additional 52 (refs 2–4) or 76–80 (refs 4,5) amino acids on the C terminus of ubiquitin, an unusual arrangement conserved in man3,5, yeast4 and plants (J. Callis and R. Vierstra, personal communication). Although not homologous to each other or to ubiquitin, both extension proteins are highly basic and contain patterns of cysteine and histidine similar to those proposed to form 'zinc fingers'6,7. The longer C-terminal extension protein (CEP80) is 30% lysine and arginine and, when denatured, behaves like a small cationic protein8. Its properties after isolation in physiological conditions, however, suggested that CEP80 is part of an RNA-protein complex. Using the antibodies that confirmed the presence of CEP80 in eukaryotic cells8, we show here that the protein is located on ribosomes. Immunoblotting of rat 40S subunit proteins specifically identifies CEP80 as ribosomal protein S27a.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Schlesinger, M. J. & Bond, U. Oxf. Surv. Euk. Genes 4, 77 (1987).
Müller-Taubenberger, A., Westphal, M., Jaeger, E., Noegel, A. & Gerisch, G. FEBS Lett. 229, 273–278 (1988).
Salvensen, G., Lloyd, C. & Farley, D. Nucleic Acids Res. 15, 5485 (1987).
Özkaynak, E., Finley, D., Solomon, M. J. & Varshavsky, A. EMBO J. 6, 1429–1439 (1987).
Lund, P. K. et al. J. biol. Chem. 260, 7609–7613 (1985).
Berg, J. M. Science 232, 485–487 (1986).
Evans, R. M. & Hollenberg, S. M. Cell 52, 1–3 (1988).
Redman, K. L. & Rechsteiner, M. J. biol. Chem. 263, 4926–4931 (1988).
Collatz, E., Wool, I. G., Lin, A. & Stöffler, G. J. biol. Chem. 251, 4666–4672 (1976).
Collatz, E. et al. J. biol. Chem. 252, 9071–9080 (1977).
Odani, S., Kenmochi, N. & Ogata, K. J. Biochemistry 103, 872–877 (1988).
Lin, A., Chan, Y.-L., Jones, R. & Wool, I. G. J. biol. Chem. 262, 14343–14351 (1987).
Cornish-Bowden, A. Analyt. Biochem. 105, 233–238 (1980).
McConkey, E. H. et al. Molec. gen. Genet. 169, 1–6 (1979).
Madjar, J.-J., Arpin, M., Buisson, M. & Reboud, J.-P. Molec. gen. Genet. 171, 121–134 (1979).
Hardy, S. J. S. & Kurland, C. G. Biochemistry 5, 3676–3684 (1966).
Nue, H. C. & Heppel, L. A. Proc. natn. Acad. Sci. U.S.A. 51, 1267–1274 (1964).
Finley, D. et al. in Ubiquitin (ed M. Rechsteiner) 39–75 (Plenum, New York), (1988).
Finley, D., Bartel, B. & Varshavsky, A. Nature (in the press).
Otaka, E., Higo, K. & Osawa, S. Biochemistry 21, 4545–4550 (1982).
Otaka, E. & Osawa, S. Molec. gen. Genet. 181, 176–182 (1981).
Rechsteiner, M. A. Rev. Cell Biol. 3, 1–30 (1987).
Nomura, M., Gourse, R. & Baughman, G. A. Rev. Biochem. 53, 75–117 (1984).
Brown, G. E., Kolb, A. J. & Stanley, W. M. Meth. Enzym 30, 368–387 (1974).
Salinovich, O. & Montelaro, R. C. Analyt. Biochem. 156, 341–347 (1986).
Madjar, J.-J., Michel, S., Cozzone, A. J. & Reboud, J.-P. Analyt. Biochem. 92, 174–182 (1979).
Fling, S. P. & Gregerson, D. S. Analyt. Biochem. 155, 83–88 (1986).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Redman, K., Rechsteiner, M. Identification of the long ubiquitin extension as ribosomal protein S27a. Nature 338, 438–440 (1989). https://doi.org/10.1038/338438a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/338438a0
This article is cited by
-
DCAF1-based PROTACs with activity against clinically validated targets overcoming intrinsic- and acquired-degrader resistance
Nature Communications (2024)
-
Myelin-associated oligodendrocytic basic protein-dependent myelin repair confers the long-lasting antidepressant effect of ketamine
Molecular Psychiatry (2023)
-
The ubiquitin thioesterase YOD1 ameliorates mutant Huntingtin induced pathology in Drosophila
Scientific Reports (2023)
-
Loss of RPS27a expression regulates the cell cycle, apoptosis, and proliferation via the RPL11-MDM2-p53 pathway in lung adenocarcinoma cells
Journal of Experimental & Clinical Cancer Research (2022)
-
Polyubiquitin gene Ubb is required for upregulation of Piwi protein level during mouse testis development
Cell Death Discovery (2021)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
