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Mitochondrial splicing requires a protein from a novel helicase family

Nature volume 337, pages 84–87 (1989)Cite this article

Abstract

Proteins involved in mitochondrial splicing but encoded by nuclear genes have been characterized in Saccharomyces and Neurospora1–5. The role in splicing of these proteins is largely unknown. Here we report that mutations in the nuclear gene MSS116 directly affect the splicing of several introns of the cytochrome b (cob) and cytochrome c oxidase subunit I (cox1) primary transcripts. This implies that the MSS116 protein (pMSS116) is an important component of the mitochondrial splicing machinery. The sequence of the cloned MSS116 gene shows that its protein product is homologous to the translation eIF-4A factor and the human nuclear protein p68. We show further that these proteins share several conserved amino-acid blocks with DNA helicases and related proteins. This suggests that pMSS116 has an RNA helicase activity. RNA helicases may be involved in many different processes including translation and splicing.

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Author notes

  1. Bertrand Séraphin

    Present address: Department of Biology, Brandeis University, Waltham, Massachusetts, 02254, USA

Authors and Affiliations

  1. Institut Curie-Biologic, Centre Universitaire, Bat. 110, 91405, Orsay Cedex, France

    Bertrand Séraphin, Michel Simon, Annick Boulet & Gérard Faye

Authors
  1. Bertrand Séraphin
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  2. Michel Simon
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  3. Annick Boulet
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  4. Gérard Faye
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Séraphin, B., Simon, M., Boulet, A. et al. Mitochondrial splicing requires a protein from a novel helicase family. Nature 337, 84–87 (1989). https://doi.org/10.1038/337084a0

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