Abstract
SV40 large T antigen contains a small region of amino acid sequence, conserved among the papovaviruses, that shows considerable similarity to conserved domain 2 of the adenovirus E1A oncogene, a domain which plays an important role in the E1A transforming functions. To learn whether the analogous SV40 T antigen sequences could substitute functionally for E1A domain 2, a chimaeric gene was constructed, coding for T antigen amino acid residues 101 to 118 in place of E1A domain 2. The resulting product showed much of the activity of the wild-type E1A products. It induced proliferation of primary BRK cells and cooperated with the ras oncogene to transform these cells fully. In addition, the chimaeric protein coprecipitated two cellular proteins whose specific binding to the E1A products depends on the presence of domain 2. The activity of the chimaeric product suggests that a similar functional unit exists in the transforming proteins of both SV40 and adenovirus, and that these proteins may exert their cell growth regulating effects through similar mechanisms.
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References
Houweling, A., van den Elsen, P. J. & van der Eb, A. J. Virology 105, 537–550 (1980).
Ruley, H. E. Nature 304, 602–606 (1983).
Moran, E. & Mathews, M. B. Cell 48, 177–178 (1987).
Kimelman, D., Miller, J. S., Porter, D. & Roberts, B. E. J. Virol. 53, 399–409 (1985).
Haley, K. P., Overhauser, G., Babiss, L. E., Ginsberg, H. S. & Jones, N. C. Proc. natn. Acad. Sci. U.S.A. 81, 5734–5738 (1984).
Montell, C., Courtois, G., Eng, C. & Berk, A. Cell 36, 951–961 (1984).
Moran, E., Grodzicker, T., Roberts, R. J., Mathews, M. B. & Zerler, B. J. Virol. 57, 765–775 (1986).
Zerler, B. et al. Molec. cell Biol. 6, 887–899 (1986).
Kuppuswamy M. N. & Chinnadurai, G. Virology 159, 31–38 (1987).
Lillie, J. W., Green, M. & Green, M. R. Cell 46, 1043–1051 (1986).
Lillie, J. W., Lowenstein, P. W., Green, M. R. & Green, M. Cell 50, 1091–1100 (1987).
Moran, E., Zerler, B., Harrison, T. M. & Mathews, M. B. Molec. cell. Biol. 6, 3470–3480 (1986).
Moran, E. & Zerler, B. Molec. cell. Biol. 8, 1756–1764 (1988).
Schneider, J. F., Fisher, F., Goding, C. R. & Jones, N. C. EMBO J. 6, 2053–2060 (1987).
Stephens, C. & Harlow, E. EMBO J. 6, 2027–2035 (1987).
Subramanian, T., Kuppuswamy, M., Nasr, Randa J. & Chinnadurai, G. Oncogene 2, 105–112 (1988).
Ulfendahl, P. J. et al. EMBO J. 6, 2037–2044 (1987).
Whyte, P., Ruley, H. E. & Harlow, E. J. Virol. 62, 257–265 (1988).
Stabel, S., Argos, P. & Philipson, L. EMBO J. 4, 2329–2336 (1985).
Quinlan, M. P. & Grodzicker, T. J. Virol. 61, 673–682 (1987).
Ralston, R. & Bishop, J. M. Nature 306, 803–806 (1983).
Figge, J., Webster, T., Smith, T. F. & Paucha, E. J. Virol. 62, 1814–1818 (1988).
Kuenzel, E., Mulligan, J. A., Sommercorn, J. & Krebs, E. G. J. biol. Chem. 262, 9136–9140 (1987).
Sommercorn, J., Mulligan, J. A., Lozeman, F. J. & Krebs, E. G. Proc. natn. Acad. Sci. U.S.A. 84, 8834–8838 (1987).
Zerler, B., Roberts, R. J., Mathews, M. B., & Moran, E. Molec. cell. Biol. 7, 821–829 (1987).
Yee, S. & Branton, P. Virology 147, 142–153 (1985).
Harlow, E., Whyte, P., Robert Franza, Jr., B. & Schley, C. Molec. cell. Biol. 6, 1579–1589 (1986).
Whyte, P., Buchkovich, K. J., Horowitz, J. M., Friend, S. H., Raybuck, M., Weinberg, R. A. & Harlow, E. Nature 334, 124–129 (1988).
DeCaprio et al. Cell (in the press).
Phelps, W. C., Yee, C. L., Munger, K. & Howley, P. M. Cell 53, 539–547 (1988).
Tokunaga, O., Yaegashi, T., Lowe, J., Dobbs, L. & Padmanabhan, R. Virology 155, 418–433 (1986).
Pawlita, M., Clad, A. & zur Hausen, H. Virology 143, 196–211 (1985).
Self, I., Khoury, G. & Dhar, R. Cell 18, 963–977 (1979).
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Moran, E. A region of SV40 large T antigen can substitute for a transforming domain of the adenovirus E1A products. Nature 334, 168–170 (1988). https://doi.org/10.1038/334168a0
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DOI: https://doi.org/10.1038/334168a0
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