Abstract
Lymphocyte function-associated antigen-1 (LFA-1) is a heterodimer composed of an α and β chain that is expressed on the surface of most leukocytes and is an essential molecule for adhesion reactions between cells participating in the immune response1,2. A putative ligand for LFA-1 is the intercellular adhesion molecule ICAM-1 (refs 3-5). Leukocyte adhesion abnormality is found in patients with LFA-1 deficiency6–7. It is not clear whether binding of ligand to the LFA-1 molecule merely spatially orientates cells towards each other or can also induce signals that regulate cell activation and differentiation. We have recently developed a T-cell proliferation assay which uses immobilized anti-CD3 monoclonal antibodies as stimulant and is independent of LFA-1-mediated cellular adhesion. As there is no interference by anti-LFA-1 monoclonal antibodies with the adhesion-dependent activation steps, this T-cell activation system allows us to investigate whether transmembrane signals are induced by binding of ligand to LFA-1 on T cells. Our data indicate that binding of ligand to LFA-1 results in the transduction of regulatory signals across the plasma membrane, rather like other molecules (CD2, CD4, CDS) (refs 8–11) with signal-modifying properties involved in the adhesion of T cells to target/stimulator cells. Indeed, adhesion molecules might generally be important in signal transduction, even in cells not belonging to the immune system.
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References
Springer, T. A., Dustin, M. L., Kishimoto, T. K. & Marlin, S. D. A. Rev. Immun. 5, 223–252 (1987).
Martz, E. Human Immun. 18, 3–37 (1987).
Rothlein, R., Dustin, M. L., Marlin, S. D. & Springer, T. A. J. Immun. 137, 1270–1274 (1986).
Makgoba, M. W. et al. Nature 331, 86–88 (1988).
Marlin, S. D. & Springer, T. A. Cell 51, 813–819 (1987).
Anderson, D. C. et al. Fedn. Proc. 44, 2671–2677 (1985).
Miedema, F. et al. J. Immun. 134, 3075–3081 (1985).
Hunig, T. R., Tiefenthaler, G., Meyer zum Büschenfelde, K. H. & Meuer, S. C. Nature 326, 298–300 (1987).
Bank, I. & Chess, L. J. exp. Med. 162, 1294–1303 (1985).
Fleisher, B., Schrezenmeier, H. & Wagner, H. J. Immun. 136, 1625–1628 (1986).
Van Seventer, G. A., Van Lier, R. A. W., Spits, H., Ivanyi, P. & Melief, C. J. M. Eur. J. Immun. 16, 1363–1371 (1986).
Van Wauwe, J. P., De May, J. R. & Goossens, J. G. J. Immun. 124, 2708–2713 (1980).
Tax, W. J. M., Hermes, F. F. M., Willems, R. W., Capel, P. J. A. & Koene, R. A. P. J. Immun. 133, 1185–1189 (1984).
Van Lier, R. A. W., De Groot, E. R., Rinnoy Kan, E. A., Zeijlemaker, W. P. & Aarden, L. A. in Tissue Typing III (eds McMichael, A. J. et al. 181 (Oxford University Press, 1987).
Geppert, T. D. & Lipsky, P. E. J. Immun. 138, 1660–1666 (1987).
Sanchez-Mardrid, F. et al. Proc. natn. Acad. Sci. U.S.A. 70, 7489–7493 (1982).
Miedema, F. et al. Eur. J. Immun. 14, 518–528 (1984).
Dougworth, D. W., Gotch, F. M., Hildreth, J. E. K., Morris, A. & McMichael, A. J. Eur. J. Immun. 15, 888–892 (1985).
Waldman, H. et al. in Tissue Typing III (eds McMichael, A. J. et al.) 829 (Oxford University Press, 1987).
Van Lier, R. A. W., Brouwer, M. & Aarden, L. A. Eur. J. Immun. 18, 167–172 (1988).
Gillis, S. et al. J. Immun. 120, 2027–2032 (1978).
Mishra, G. C. et al. J. Immun. 137, 1590–1598 (1986).
Pircher, H. et al. Eur. J. Immun. 16, 172–181 (1986).
Ross, G. D. & Medof, M. D. Adv. Immun. 37, 217–267 (1985).
Tsoukas, C. D., Valentine, M., Lotz, M., Vaughan, J. H. & Carson, D. A. Immun. Today 5, 311–313 (1984).
Ruoslahti, E. & Pierschbacher, M. D. Science 238, 491–497 (1987).
Gallatin, M. et al. Cell 44, 673–680 (1986).
Cunningham, B. A. TIBS 11, 423–426 (1986).
Kishimoto, T. K., O'Connor, K., Lee, A., Roberts, T. M. & Springer, T. A. Cell 48, 681–690 (1987).
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van Noesel, C., Miedema, F., Brouwer, M. et al. Regulatory properties of LFA-1 α and β chains in human T-lymphocyte activation. Nature 333, 850–852 (1988). https://doi.org/10.1038/333850a0
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DOI: https://doi.org/10.1038/333850a0
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