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  • Viral Transfer Technology
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Clinical retroviral vector production: step filtration using clinically approved filters improves titers

Gene Therapy volume 7, pages 1993–1998 (2000)Cite this article

Abstract

Production of retroviral vectors for clinical use requires removal of cells and cellular debris. We combined a series of filters of decreasing pore size using commercially available blood banking filters approved for clinical use. The collection bag and filters can be connected to create a sterile, closed system using clinically approved tubing and sealing systems. Even when challenged with a large number of vector producer cells (2.38 × 109cells), no viable cells are passed through the system. The step filtration system developed minimizes the titer reduction associated with filtration, provides rapid flow rates, and was cost effective when filtering volumes in excess of 2 liters.

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Acknowledgements

We are indebted to Dr Philip Maples for his technical advice in filter selection. We would like to thank Christine Starkey, Clara Hazelgrove and Lisa Woodsides for their technical contributions to this manuscript. The Indiana University Vector Production Facility is a NIH designated National Gene Vector Laboratory (U42 RR11148) and this work was supported in part by a Centers of Excellence in Molecular Hematology grant (PHS P50 DK49218) and a core laboratory supporting PHS P01 HL53586.

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  1. Division of Hematology/Oncology, Departments of Medicine, Medical and Molecular Genetics, and Microbiology/Immunology, Indiana University School of Medicine, Indianapolis, IN, USA

    L Reeves & K Cornetta

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  1. L Reeves
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  2. K Cornetta
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Reeves, L., Cornetta, K. Clinical retroviral vector production: step filtration using clinically approved filters improves titers. Gene Ther 7, 1993–1998 (2000). https://doi.org/10.1038/sj.gt.3301328

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