Abstract
When a chimaeric soybean leghaemoglobin gene was introduced into the genome of another legume species, Lotus corniculatus, nodule-specific expression of the chimaeric gene was found in root nodules formed on fully regenerated plants inoculated with the Lotus microsymbiont, Rhizobium loti. Expression under control of the 5′ upstream region of the soybean gene was regulated at the level of RNA and followed the correct developmental timing. This indicates a conserved induction mechanism for leghaemoglobin genes in legumes.
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References
Vincent, J. M. in The Biology of Nitrogen Fixation (ed. Quispel, A.) 265–341 (North-Holland, Amsterdam, 1974).
Vincent, J. M. in Nitrogen Fixation II (eds Newton, W. E. & Orme Johnson, W. H.) 103–129 (University Park, Baltimore, 1980).
Legocki, R. P. & Verma, D. P. S. Cell 20, 153–163 (1980).
Bisseling, T., Been, C., Klugkist, J., Van Kammen, A. & Nadler, K. EMBO J. 2, 961–966 (1983).
Cullimore, J. V. et al. J. molec. appl. Genet. 2, 589–599 (1984).
Jochimsen, B. & Rasmussen, O. in Abstr. 1st int. Symp. Molecular Genetics of the Bacteria-Plant Interactions, 83 (Springer, Berlin, 1982).
Nguyen, T., Zelechowska, M., Foster, V., Bergmann, H. & Verma, D. P. S. Proc. natn. Acad. Sci. U.S.A. 82, 5040–5044 (1985).
Fuchsman, W. H. & Appleby, C. A. Biochim. biophys. Acta 579, 314–324 (1979).
Bojsen, K., Abildsten, D., Jensen, E. Ø., Paludan, K. & Marcker, K. A. EMBO J. 2, 1165–1168 (1983).
Lee, J. S., Brown, G. G. & Verma, D. P. S. Nucleic Acids Res. 11, 5541–5553 (1983).
Marcker, A., Lund, M., Jensen, E. Ø. & Marcker, K. A. EMBO J. 3, 1691–1695 (1984).
Grosveld, G. C., de Boer, E., Shewmaker, C. K. & Flavell, R. A. Nature 295, 120–126 (1982).
Dierks, P. et al. Cell 32, 695–706 (1983).
Wright, S., Rosenthal, A., Flavell, R. & Grosveld, F. Cell 38, 265–273 (1984).
Leemans, J. et al. J. molec. appl. Genet. 1, 149–164 (1981).
Van Haute, E. et al. EMBO J. 3, 411–417 (1983).
White, F. F. & Nester, E. W. J. Bact. 141, 1134–1141 (1980).
Chilton, M.-D. et al. Nature 295, 432–434 (1982).
Pankhust, C. E. Can. J. Microbiol. 23, 1026–1033 (1977).
Wiborg, O. et al. EMBO J. 4, 755–759 (1985).
Causing, K. & Barkardottir, R. Eur. J. Biochem. (in the press).
Brissan, N. & Verma, D. P. S. Proc. natn. Acad. Sci. U.S.A. 79, 4055–4059 (1982).
Nienhuis, A. W. & Benz, E. J. New Engl. J. Med. 297, 1318–1328; 1371–1381, 1430–1436 (1977).
Marton, L., Wullems, G. J., Molendijk, L. & Schilperoort, R. A. Nature 277, 129–131 (1979).
Horsch, R. B. et al. Science 227, 1229–1231 (1985).
Pollock, K., Barfield, D. G., Robinson, S. J. & Shields, R. Pl. Cell Rep. 4, 202–205 (1985).
Zambryski, P. et al. EMBO J. 2, 2143–2150 (1983).
Herrera-Estrella, L. et al. EMBO J. 2, 987–995 (1983).
De Block, M., Herrera-Estrella, L., Van Montagu, M., Schell, J. & Zambryski, P. EMBO J. 3, 1681–1689 (1984).
Sengupta-Gopalan, C., Reichert, N. A., Barker, R. F., Hall, T. L. & Kemp, J. O. Proc. natn. Acad. Sci. U.S.A. 82, 3320–3324 (1985).
Lamppa, G., Nagy, F. & Chua, N.-H. Nature 316, 750–752 (1985).
De Block, M., Schell, J. & Van Montagu, M. EMBO J. 4, 1367–1372 (1985).
Simpson, J. et al. EMBO J. 4, 2723–2729 (1985).
Nagy, F., Morelli, G., Graley, S. G. & Chua, N.-H. EMBO J. 4, 3063–3068 (1985).
Beachy, R. N. et al. EMBO J. 4, 3047–3053 (1985).
Jones, J. D. G., Dunsmuir, P. & Bedbrook, J. EMBO J. 4, 2411–2418 (1985).
Jensen, E. Ø., Marcker, K. A. & Villadsen, I. S. EMBO J. 5 (in the press).
Herrea-Estrella, L. et al. Nature 310, 115–120 (1984).
Morelli, G., Nagy, F., Fraley, R. T., Rogers, S. G. & Chua, N.-H. Nature 315, 200–204 (1985).
Facciotti, D., O'Neal, J. K., Lee, S. & Shewmaker, C. H. Biotechnology 3, 241–246 (1985).
Timko, M. P. et al. Nature 318, 579–582 (1985).
Polhill, R. M. in Advances in Legume Systematics Vol. 1 (eds Polhill, R. M. & Raven, D. H.) (University of Reading, 1981).
Trinick, M. J. J. appl. Bact. 49, 39–53 (1980).
Dhaese, P., De Greve, H., Decraemer, H., Schell, J. & Van Montagu, M. Nucleic Acids Res. 7, 1837–1849 (1979).
Pomponi, M., Spano, L., Sabbadini, M. G. & Costantino, P. Plasmid 10, 119–129 (1983).
Dellaporta, S. L., Wood, J. & Hicks, J. B. Pl. molec. Biol. Rep. 1, 10–21 (1983).
Boel, E. et al. Proc. natn. Acad. Sci. U.S.A. 80, 2866–2869 (1983).
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Jensen, J., Marcker, K., Otten, L. et al. Nodule-specific expression of a chimaeric soybean leghaemoglobin gene in transgenic Lotus corniculatus. Nature 321, 669–674 (1986). https://doi.org/10.1038/321669a0
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DOI: https://doi.org/10.1038/321669a0
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