Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

A cDNA clone encoding an S-locus-specific glycoprotein from Brassica oleracea

Nature volume 318, pages 263–267 (1985)Cite this article

A Corrigendum to this article was published on 01 April 1987

This article has been updated

Abstract

The pollen–stigma interaction resulting in self-incompatibility in the cruciferous plant Brassica has been correlated with glycoproteins that display polymorphisms in all S genotypes examined. A complementary DNA clone encoding these glycoproteins has been isolated and characterized. Polymorphisms in homologous restriction fragments of Brassica genomic DNA segregate precisely with alleles at the S locus.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

Change history

References

  1. East, E.M. Proc. Am. phil. Soc. 82, 449–518 (1940).

    Google Scholar 

  2. Thompson, K.F., & Taylor, J.P. Heredity 21, 345–362 (1966).

    Article  Google Scholar 

  3. Ockendon, D.J. Heredity 33, 159–171 (1974).

    Article  Google Scholar 

  4. Nasrallah, M.E. & Wallace, D.H. Heredity 22, 519–527 (1967).

    Article  Google Scholar 

  5. Nasrallah, M.E., Barber, J.T. & Wallace, D.H. Heredity 25, 23–27 (1970).

    Article  Google Scholar 

  6. Nasrallah, M.E., Wallace, D.H. & Savo, R.M. Genet. Res. 20, 151–160 (1972).

    Article  Google Scholar 

  7. Nishio, T. & Hinata, K. Heredity 38, 391–396 (1977).

    Article  Google Scholar 

  8. Nasrallah, J.B. & Nasrallah, M.E. Experientia 40, 279–281 (1984).

    Article  CAS  Google Scholar 

  9. Nasrallah, J.B., Doney, R.C. & Nasrallah, M.E. Planta 165, 100–107 (1985).

    Article  CAS  Google Scholar 

  10. Nasrallah, M.E. Genetics 76, 45–50 (1974).

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Hinata, K. & Nishio, T. Heredity 41, 93–100 (1978).

    Article  Google Scholar 

  12. Taylor, J.M., Illmensee, R. & Summers, J. Biochim. biophys. Acta 442, 324–330 (1976).

    Article  CAS  Google Scholar 

  13. Maniatis, T., Fritsch, E.F. & Sambrook, J. Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory, New York, 1982).

    Google Scholar 

  14. Grunstein, M. & Hogness, D.S. Proc. natn. Acad. Sci. U.S.A. 72, 3961–3965 (1975).

    Article  ADS  CAS  Google Scholar 

  15. Sanger, F., Coulson, A.R., Barrell, B.G., Smith, A.J.H. & Rose, B. J. molec. Biol. 143, 161–178 (1980).

    Article  CAS  Google Scholar 

  16. Proudfoot, N.J. & Brownbee, G.G. Nature 263, 211–214 (1976).

    Article  ADS  CAS  Google Scholar 

  17. Ullrich, A. et al. Nature 309, 418–425 (1984).

    Article  ADS  CAS  Google Scholar 

  18. Neuberger, A., Marshall, R.D. & Spiro, R. G. in The Glycoproteins: Their Composition, Structure and Function Pt A (ed. Gottschalk, A.) 450–490 (Elsevier, Amsterdam, 1972).

    Google Scholar 

  19. Guo, L.H. et al. Gene 29, 251–254 (1984).

    Article  CAS  Google Scholar 

  20. Nishio, T. & Hinata, K. Genetics 100, 644–647 (1982).

    Google Scholar 

  21. Hall, T.C. et al. in Genome Organization and Expression in Higher Plants (ed. Leaver, C.) 259–272 (Plenum, New York, 1980).

    Book  Google Scholar 

  22. Beachy, R.N., Barton, K.A., Madison, J.T., Thompson, J.F. & Jarvis, N. in Genome Organization and Expression in Higher Plants (ed. Leaver, C.) 273–281 (Plenum, New York, 1980).

    Book  Google Scholar 

  23. Thomas, P.S. Proc. natn. Acad. Sci. U.S.A. 77, 5201–5205 (1980).

    Article  ADS  CAS  Google Scholar 

  24. Rigby, P.W.J., Dieckmann, M., Rhodes, C. & Berg, P. J. molec. Biol. 113, 237–251 (1977).

    Article  CAS  Google Scholar 

  25. Messing, J. & Vieira, J. Gene 19, 269–276 (1982).

    Article  CAS  Google Scholar 

  26. Hanahan, D.J. molec. Biol. 166, 557–580 (1983).

    Article  CAS  Google Scholar 

  27. Laemmli, U.K. Nature 227, 680–685 (1970).

    Article  ADS  CAS  Google Scholar 

  28. Towbin, H., Staehlin, T. & Gordon, J. Proc. natn. Acad. Sci. U.S.A. 76, 4350–4354 (1979).

    Article  ADS  CAS  Google Scholar 

  29. Symington, J. in Two-Dimensional Electrophoresis of Proteins (eds Celis, J. & Bravo, R.) 129–168 (Academic, New York, 1983).

    Google Scholar 

  30. Linskens, H.F. & Esser, K.L. Naturwissenschaften 44, 1–2 (1957).

    Article  ADS  Google Scholar 

  31. Martin, F.W. Stain Techn. 34, 125–128 (1959).

    Article  CAS  Google Scholar 

  32. Southern, E.J. J. molec. Biol. 98, 503–517 (1975).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Section of Plant Biology, Cornell University, Ithaca, New York, 14853, USA

    J. B. Nasrallah & M. E. Nasrallah

  2. Sections of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York, 14853, USA

    T.-H. Kao

  3. Section of Genetics and Development, Cornell University, Ithaca, New York, 14853, USA

    M. L. Goldberg

Authors
  1. J. B. Nasrallah
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T.-H. Kao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. L. Goldberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. E. Nasrallah
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nasrallah, J., Kao, TH., Goldberg, M. et al. A cDNA clone encoding an S-locus-specific glycoprotein from Brassica oleracea. Nature 318, 263–267 (1985). https://doi.org/10.1038/318263a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/318263a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing