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Mutations that alter the DNA sequence specificity of the catabolite gene activator protein of E. coli

Nature volume 311, pages 232–235 (1984)Cite this article

Abstract

Three mutations that alter the DNA sequence specificity of the catabolite gene activator protein (CAP) from AA-TGTGA--T---TCA-ATW to AA-TGTAA--T---TCA-ATW have been isolated. All three mutations affect the same amino acid of CAP, glutamic acid 181. We propose that it is this amino acid of CAP that makes contacts with base pairs 7 and 16 of the symmetrical recognition site.

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Author information

Authors and Affiliations

  1. Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts, 02115, USA

    Richard H. Ebright & Jon Beckwith

  2. Unité de Biochimie Cellulaire, Institut Pasteur, 28 Rue du Dr Roux, 75724, Paris, Cedex 15, France

    Pascale Cossart

  3. Unité de Biochimie des Regulations Cellulaires, Institut Pasteur, 28 Rue du Dr Roux, 75724, Paris, Cedex 15, France

    Brigitte Gicquel-Sanzey

Authors
  1. Richard H. Ebright
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  2. Pascale Cossart
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  3. Brigitte Gicquel-Sanzey
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  4. Jon Beckwith
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Ebright, R., Cossart, P., Gicquel-Sanzey, B. et al. Mutations that alter the DNA sequence specificity of the catabolite gene activator protein of E. coli. Nature 311, 232–235 (1984). https://doi.org/10.1038/311232a0

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