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Correct transcription of an immunoglobulin κ gene requires an upstream fragment containing conserved sequence elements

Nature volume 310, pages 71–74 (1984)Cite this article

Abstract

Transcription of the immunoglobulin κ light-chain genes depends on the presence of a TATA box upstream of the leader gene segment1,2 and is regulated by an enhancer sequence in the large intron3,4. In studying a rearranged mouse κ light-chain gene we have now found that sequences between −90 and −160 base pairs (bp) upstream of the coding region are essential for correct transcription in gene transfer experiments. This region contains the deca- and pentadecanucleotide sequences TNATTTGCAT and TGCAGCTGTGNCCAG, which we call dc and pd, respectively. Sequences related to dc and pd were found upstream of all human and mouse κ-chain variable region (Vκ) genes, upstream of λ-chain variable region (Vλ) genes, and within the mouse heavy-chain enhancer5,6. An inverted and complementary form of the dc element (ATGCAAATNA, called cd) occurs upstream of all heavy-chain variable region (VH) genes. The newly defined sequences may be involved in the control of immunoglobulin gene transcription.

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Authors and Affiliations

  1. Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie der Universität München, 8000, München, 2, FRG

    F. G. Falkner & H. G. Zachau

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  1. F. G. Falkner
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  2. H. G. Zachau
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Falkner, F., Zachau, H. Correct transcription of an immunoglobulin κ gene requires an upstream fragment containing conserved sequence elements. Nature 310, 71–74 (1984). https://doi.org/10.1038/310071a0

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