Abstract
African trypanosomes activate, one at a time, a large set of genes coding for different variant-specific surface antigens (VSAs). These genes have been classed into two groups. In the first group a permanently silent basic gene copy is duplicated and the expression-linked copy (ELC) transposed to an expression site located at a chromosome end1–3. The process is a gene conversion which changes a variable stretch of the preceding ELC4. Genes belonging to the second group do not give rise to an additional copy when expressed by a still unknown mechanism5,6. We report here that the gene for antigenic type AnTat 1.6 is located in a telomeric DNA region and is expressed without being duplicated. In clone AnTat 1.6 and the ensuing ones, the ELC of the preceding VSA (AnTat 1.3) is conserved, but in a inactive conformation. Moreover, the AnTat 1.6 gene is lost from the genome of the AnTat 1.6-derived variants, in which the duplication-linked mechanism of gene activation occurs: the gene appears to be replaced by the incoming ELC. These observations show that a trypanosome surface antigen repertoire may evolve by loss and gain of VSA genes, depending on the alternation of the different recombinational mechanisms involved in antigenic variation.
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References
Hoeijmakers, J. H. J., Frasch, A. C. C., Bernards, A., Borst, P. & Cross, G. A. M. Nature 284, 78–80 (1980).
Pays, E., Van Meirvenne, N., Le Ray, D. & Steinert, M. Proc. natn. Acad. Sci. U.S.A. 78, 2673–2677 (1981).
Bernards, A. et al. Cell 27, 497–505 (1981).
Pays, E. et al. Cell, 34, 371–381 (1983).
Young, J. R., Donelson, J. E., Majiwa, P. A. O., Shapiro, S. Z. & Williams, R. O. Nucleic Acids Res. 10, 803–819 (1982).
Majiwa, P. A. O., Young, J. R., Englund, P. T., Shapiro, S. Z. & Williams, R. O. Nature 297, 514–516 (1982).
Laurent, M. et al. Nature 302, 263–266 (1983).
Pays, E. et al. Cell 34, 357–369 (1983).
Borst, P. & Cross, G. A. M. Cell 29, 291–303 (1982).
Williams, R. O., Young, J. R. & Majiwa, P. A. O. Nature 299, 417–421 (1982).
Young, J. R., Shah, J. S., Matthyssens, G. & Williams, R. O. Cell 32, 1149–1159 (1983).
Bernards, A., Michels, P. A. M., Lincke, C. R. & Borst, P. Nature 303, 592–597 (1983).
Pays, E. et al. Nucleic Acids Res. 8, 5965–5981 (1980).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
Weintraub, H. & Groudine, M. Science 193, 848–856 (1976).
Pays, E. et al. Cell 35, 721–731 (1983).
Le Ray, D., Barry, J. D., Easton, C. & Vickerman, K. Ann. Soc. belge Méd. trop. 57, 369–381 (1977).
Le Ray, D., Barry, D. & Vickerman, K. Nature 273, 300–302 (1978).
Hadjuk, S. L., Cameron, C. R., Barry, J. D. & Vickerman, K. Parasitology 83, 595–607 (1981).
Van Meirvenne, N., Janssens, P. G. & Magnus, E. Ann. Soc. Belge Méd. trop. 55, 1–23 (1975).
Pays, E., Lheureux, M. & Steinert, M. Nature 292, 265–267 (1981).
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Laurent, M., Pays, E., Delinte, K. et al. Evolution of a trypanosome surface antigen gene repertoire linked to non-duplicative gene activation. Nature 308, 370–373 (1984). https://doi.org/10.1038/308370a0
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DOI: https://doi.org/10.1038/308370a0
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