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Effect of chromosomal position on amplification of transfected genes in animal cells

Nature volume 307, pages 516–520 (1984)Cite this article

Abstract

A single protein (CAD) contains the first three enzymatic activities of de novo undine biosynthesis. The chromosomal location of CAD genes introduced into Chinese hamster ovary cells significantly affects the frequency and cytogenetic result of their amplification. The amplification frequency in one transformant is 100-fold that of the others; in another, amplification of donated genes inserted near a centromere results in chromosome instability and rearrangements.

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Author notes

  1. Bruno Robert de Saint Vincent

    Present address: The Pasteur Institute, 25 Rue du Dr Roux, Cedex 7595, France

Authors and Affiliations

  1. The Salk Institute, PO Box 85800, San Diego, California, 92138, USA

    Geoffrey M. Wahl, Bruno Robert de Saint Vincent & Margaret L. DeRose

Authors
  1. Geoffrey M. Wahl
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  2. Bruno Robert de Saint Vincent
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  3. Margaret L. DeRose
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Wahl, G., Robert de Saint Vincent, B. & DeRose, M. Effect of chromosomal position on amplification of transfected genes in animal cells. Nature 307, 516–520 (1984). https://doi.org/10.1038/307516a0

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