Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Optical recording of action potentials from vertebrate nerve terminals using potentiometric probes provides evidence for sodium and calcium components

Nature volume 306, pages 36–40 (1983)Cite this article

Abstract

Optical methods are shown to monitor action potentials from a population of nerve terminals in the neurohypophysis of Xenopus. Calcium antagonists such as cadmium and nickel ions block a component of the action potential that probably reflects a calcium-mediated potassium conductance, and tetrodotoxin blocks an inward sodium current, revealing a calcium component to the action potential upstroke.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Bullock, T. H. & Hagiwara, S. J. gen. Physiol. 40, 565–577 (1957).

    Article  CAS  Google Scholar 

  2. Katz, B. & Miledi, R. J. Physiol., Lond. 192, 407–436 (1967).

    Article  CAS  Google Scholar 

  3. Llinas, R., Steinberg, I. Z. & Walton, K. Proc. natn. Acad. Sci. U.S.A. 73, 2918–2922 (1976).

    Article  ADS  CAS  Google Scholar 

  4. Katz, B. The Release of Neural Transmitter Substances (Thomas, Springfield, 1969).

    Google Scholar 

  5. Douglas, W. W. Nature 197, 81–82 (1963).

    Article  ADS  CAS  Google Scholar 

  6. Douglas, W. W. & Poisner, A. M. J. Physiol., Lond. 172, 1–18 (1964).

    Article  CAS  Google Scholar 

  7. Dreifuss, J. J., Kalnins, I., Kelley, J. S. & Ruf, K. B. J. Physiol., Lond. 215, 805–817 (1971).

    Article  CAS  Google Scholar 

  8. Cohen, L. B. & Salzberg, B. M. Rev. Physiol. Biochem. Pharmac. 83, 33–88 (1978).

    Google Scholar 

  9. Waggoner, A. S. A. Rev. Biophys. Bioengng 8, 47–68 (1979).

    Article  CAS  Google Scholar 

  10. Salzberg, B. M. in Cellular Neurophysiology (eds Barker, J. & McKelvy, J.) (Wiley, New York, in the press).

  11. Blaustein, M. P. & Goldring, J. M. J. Physiol., Lond. 247, 589–615 (1975).

    Article  CAS  Google Scholar 

  12. Kamino, K. & Inouye, A. Jap. J. Physiol. 28, 225–237 (1978).

    Article  CAS  Google Scholar 

  13. Grinvald, A. & Farber, I. Science 212, 1164–1167 (1981).

    Article  ADS  CAS  Google Scholar 

  14. Meech, R. W. & Strumwasser, F. Fedn Proc. 29, 834 (1970).

    Google Scholar 

  15. Salzberg, B. M., Davila, H. V. & Cohen, L. B. Nature 246, 508–509 (1973).

    Article  ADS  CAS  Google Scholar 

  16. Nordmann, J. J. J. Anat. 123, 213–218 (1977).

    CAS  PubMed  PubMed Central  Google Scholar 

  17. Rodriguez, E. M. & Dellman, H. D. Gen. comp. Endocr. 15, 272–288 (1970).

    Article  CAS  Google Scholar 

  18. Dellman, H. D. Int. Rev. Cytol. 36, 215–315 (1973).

    Article  Google Scholar 

  19. Gerschenfeld, H. M., Tramezzani, J. H. & De Robertis, E. Endocrinology 66, 741–762 (1960).

    Article  CAS  Google Scholar 

  20. Stampfli, R. & Hille, B. in Frog Neurobiology (eds Linas, R. & Precht, W.) 3–32 (Springer, Berlin, 1976).

    Book  Google Scholar 

  21. Poulain, D. A. & Wakerley, J. B. Neuroscience 7, 773–808 (1982).

    Article  CAS  Google Scholar 

  22. Barrett, J. N., Magleby, K. L. & Palotta, B. S. J. Physiol., Lond. 331, 211–230 (1982).

    Article  CAS  Google Scholar 

  23. Llinas, R. & Sugimori, M. J. Physiol., Lond. 305, 171–195 (1980).

    Article  CAS  Google Scholar 

  24. Llinas, R. and Sugimori, M. J. Physiol., Lond. 305, 197–213 (1980).

    Article  CAS  Google Scholar 

  25. Standen, N. B. Nature 293, 158–159 (1981).

    Article  ADS  CAS  Google Scholar 

  26. Kaufmann, R. & Fleckenstein, A. Pflügers Arch. ges. Physiol. 282, 290–297 (1965).

    Article  CAS  Google Scholar 

  27. Douglas, W. W. & Taraskevich, P. S. J. Physiol., Lond. 309, 623–630 (1980).

    Article  CAS  Google Scholar 

  28. Taraskevich, P. S. & Douglas, W. W. Nature 276, 832–834 (1978).

    Article  ADS  CAS  Google Scholar 

  29. Katz, B. & Miledi, R. J. Physiol., Lond. 203, 459–498 (1969).

    Article  CAS  Google Scholar 

  30. Douglas, W. W. in Handbook of Physiology Sect. 7, Vol. 6 (eds Blaschko, H., Sayers, G. & Smith, A. D.) 367–388 (American Physiological Society, Washington DC, 1975).

    Google Scholar 

  31. Brandt, B. L., Hagiwara, S., Kidokoro, Y. & Miyazaki, S. J. Physiol., Lond. 263, 417–439 (1976).

    Article  CAS  Google Scholar 

  32. Kamino, K., Hirota, A. & Fujii, S. Nature 290, 595–597 (1981).

    Article  ADS  CAS  Google Scholar 

  33. Gupta, R. K. et al. J. Membrane Biol. 58, 123–137 (1981).

    Article  CAS  Google Scholar 

  34. Salzberg, B. M. et al. J. Neurophysiol. 40, 1281–1291 (1977).

    Article  CAS  Google Scholar 

  35. Grinvald, A., Cohen, L. B., Lesher, S. & Boyle, M. B. J. Neurophysiol. 45, 829–840 (1981).

    Article  CAS  Google Scholar 

  36. Grinvald, A., Manker, A. & Segal, M. J. Physiol., Lond. 333, 269–291 (1982).

    Article  CAS  Google Scholar 

  37. Senseman, D. M., Shimizu, H., Horwitz, I. S. & Salzberg, B. M., J. gen. Physiol. 81, 887–908 (1983).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Physiology and Pharmacology, School of Dental Medicine, and Department of Physiology, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, 19104

    B. M. Salzberg, A. L. Obaid, D. M. Senseman & H. Gainer

  2. Laboratory of Neurochemistry and Neuroimmunology, National Institutes of Health, Bethesda, Maryland, 20205

    B. M. Salzberg, A. L. Obaid, D. M. Senseman & H. Gainer

  3. Marine Biological Laboratory, Woods Hole, Massachusetts, 02543, USA

    B. M. Salzberg, A. L. Obaid, D. M. Senseman & H. Gainer

Authors
  1. B. M. Salzberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. L. Obaid
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. M. Senseman
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. H. Gainer
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Salzberg, B., Obaid, A., Senseman, D. et al. Optical recording of action potentials from vertebrate nerve terminals using potentiometric probes provides evidence for sodium and calcium components. Nature 306, 36–40 (1983). https://doi.org/10.1038/306036a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/306036a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing