Rapid transport of foreign particles microinjected into crab axons

Abstract

The rapid transport of optically detectable organelles in axons has been well documented, although its molecular mechanism remains unknown^1–3. Here we report that synthetic particles microinjected into the giant axons of the shore crab, Carcinus maenas, are also transported, moving as though they were endogenous organelles. Polystyrene beads, polyacrolein beads, paraffin droplets and glass fragments, of sizes up to 0.5 µm in diameter, have been tested. Many of these foreign particles move rapidly and for long distances along the axon in the anterograde direction, travelling in a saltatory fashion, within a well defined velocity range. In many respects the movements are indistinguishable from those of anterogradely moving endogenous organelles seen by phase-contrast in these axons. Our results indicate that there is a transport system in axons capable of carrying almost any particle of suitable physical properties in an anterograde direction.