Abstract
The production of stable T-cell clones is essential for the study of T-cell-derived, specific immunoregulatory products and of specific T-cell receptors. T-cell clones have been established by radiation leukaemia virus (RadLV)-induced transformation of suppressor T lymphocytes specific for hen egg white lysozyme (HEL). We report here that culture supernatant obtained from these T-cell clones can, when injected into mice, specifically suppress the anti-HEL antibody response. This monoclonal T-cell product suppresses the antibody response induced by HEL and human lysozyme, but not that induced by ring-necked pheasant egg white lysozyme (REL), thus displaying fine antigenic specificity probably restricted to an epitope involving phenylalanine at amino acid residue 3, present in the N-terminal region of HEL and shared by human lysozyme but absent in REL. The suppression induced by this monoclonal T-cell product is restricted by both H–2 and Igh–1 genes whereas anti-HEL antibodies bearing a predominant idiotype are induced in all mice strains tested, irrespective of their H–2 haplotype or Igh–1 allotype1.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Benjamin, C. D., Miller, A., Sercarz, E. E. & Harvey, M. A. J. Immun. 125, 1017–1025 (1980).
Sercarz, E. E. & Metzger, D. W. Springer Semin. Immunopath. 3, 145–170 (1980).
Hill, S. W. & Sercarz, E. E. Eur. J. Immun. 5, 317–324 (1975).
Adorini, L., Miller, A. & Sercarz, E. E. J. Immun. 122, 871–877 (1979).
Finn, O.J., Boniver, J. & Kaplan, H.S. Proc. natn. Acad. Sci. U.S.A. 76, 4033–4037 (1979).
Ricciardi-Castagnoli, P., Doria, G. & Adorini, L. Proc. natn. Acad. Sci. U.S.A. 78, 3804–3808 (1981).
Ricciardi-Castagnoli, P., Robbiati, F., Barbanti, E., Doria, G. & Adorini, L. Curr. Topics Microbiol. Immun. 100, 89–96 (1982).
Ricciardi-Castagnoli, P., Lieberman, M., Finn, O. J. & Kaplan, H. S. J. exp. Med. 148, 1292–1310 (1978).
Adorini, L., Doria, G. & Ricciardi-Castagnoli, P. Eur. J. Immun. 9, 719–724 (1982).
Adorini, L., Harvey, M. A., Rozyka-Jackson, D., Miller, A. & Sercarz, E. E. J. exp. Med. 152, 521–531 (1980).
Kurosawa, Y. et al. Nature 290, 565–570 (1981).
Nakanishi, K. et al. Proc. natn. Acad. Sci. U.S.A. 79, 6984–6988 (1982).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Adorini, L., Pini, C., De Santis, R. et al. Monoclonal suppressor T-cell factor displaying VH restriction and fine antigenic specificity. Nature 303, 704–706 (1983). https://doi.org/10.1038/303704a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/303704a0
This article is cited by
-
The immune system and lymphoma
La Ricerca in Clinica e in Laboratorio (1987)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
