Abstract
Immunoglobulin variable (V) gene regions1–10 typify extensive multigenic families in terms of overall size, chromosomal arrangement and presence of large numbers of apparent pseudogenes. A unique mechanism of somatic reorganization involving recombination of VH, D and JH or VL and JL segments accompanies the differentiation of lymphoid cells5,6,11,12 and together with somatic mutation6,13,14 and other types of recombination15,16 accounts for V-region diversity. Although these processes have been well characterized in higher mammals, little is known concerning their origin and diversification during phylogenetic time. Previously, we described the blot-hybridization characteristics of murine VHIII probes with restriction enzyme-digested genomic DNA isolated from several phylogenetically critical species, including Caiman crocodylus, a modern representative of an ancient reptilian subclass17. Here we have used a murine probe, S107V, to select homologous clones from a library of Caiman genomic DNA constructed in a λ bacteriophage. The complete nucleotide sequence of a Caiman gene homologous to the murine VH gene and its adjacent 5′ and 3′ region is described. Comparison of the sequence with mammalian prototypes shows evidence of considerable organizational and structural homology extending outside the presumed VH-coding region and including elements believed to be involved in somatic recombination. Inferences about the evolution of this multigenic family can now be extended to the level of phylogenetic class.
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Litman, G., Berger, L., Murphy, K. et al. Complete nucleotide sequence of an immunoglobulin VH gene homologue from Caiman, a phylogenetically ancient reptile. Nature 303, 349–352 (1983). https://doi.org/10.1038/303349a0
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DOI: https://doi.org/10.1038/303349a0
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