Abstract
Following the discovery of the growth hormone release-inhibiting factor somatostatin from extracts of ovine hypothalamus1, an N-terminally extended somatostatin of 28 amino acids has been identified in mammalian tissue2–6. The original peptide, somatostatin-14 (SS14), corresponds to the C-terminus of somatostatin-28 (SS28). Both SS28 and SS14 have biological activity3,7–9, occur in several rat brain regions10–15, are present in cell bodies and nerve terminals11–14,16 and can be released in vitro upon depolarization in a calcium-dependent manner16–19. Further, high-affinity binding sites were described for SS14, which also bind SS28 (refs 20–23). Recently, a dodecapeptide which corresponds to the N-terminus of somatostatin-28, somatostatin-28(1–12), has been characterized in rat hypothalamus24. Radioimmunological25 and immunohistochemical13 studies have indicated the presence of SS28(1–12)-like immunoreactivity in several cortical and subcortical regions of the rat brain. This peptide was found to be unevenly distributed with the highest concentration in the hypothalamus, and preferentially localized to dendritic and axonal processes and terminals. These observations suggest that SS28(1–12) may be a neurotransmitter. In this study, we describe a calcium-dependent release of a SS28(1–12)-like peptide from hypothalamic slices in vitro. This finding supports a neurotransmitter function for this peptide.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Brazeau, P. et al. Science 179, 77–79 (1973).
Pradayrol, L., Jornvall, H., Mutt, V. & Ribet, A. FEBS Lett. 109, 55–58 (1980).
Schally, A. V. et al. Proc. natn. Acad. Sci. U.S.A. 77, 4489–4493 (1980).
Esch, F. et al. Proc. natn. Acad. Sci. U.S.A. 77, 6827–6831 (1980).
Spiess, J., Villareal, J. & Vale, W. Biochemistry 20, 1982–1988 (1981).
Bohlen, P., Brazeau, P., Esch, F., Ling, N. & Guillemin, R. Regulat. Peptides 2, 359–369 (1981).
Renaud, L. P., Martin, J. B. & Brazeau, P. Nature 255, 233–235 (1975).
Ioffe, S., Havlicek, V., Friesen, H. & Chernick, V. Brain Res. 153, 414–418 (1978).
Brazeau, P. et al. C.r. hebd. Séanc. Acad. Sci., Paris 290, 1369–1371 (1980).
Brownstein, M., Arimura, A., Sato, H., Schally, A. V. & Kizer, J. S. Endocrinology 96, 1456–1461 (1975).
Hokfelt, H. et al. Neuroscience 1, 131–136 (1976).
Epelbaum, J., Brazeau, P., Tsang, D., Brawer, J. & Martin, J. B. Brain Res. 126, 309–323 (1977).
Morrison, J. H., Benoit, R., Magistretti, P. J. & Bloom, F. E. Brain Res. (in the press).
Benoit, R., Ling, N., Alford, B. & Guillemin, R. Biochem. biophys. Res. Commun. 107, 944–950 (1982).
Kobayashi, R. M., Brown, M. & Vale, W. Brain Res. 126, 584–588 (1977).
Kewley, C. F., Millar, R. P., Berman, M. C. & Schally, A. V. Science 213, 913–915 (1981).
Iversen, L. L. et al. Nature 273, 161–163 (1978).
Bennett, G. W. et al. J. Neurochem. 32, 1127–1130 (1979).
Peterfreund, R. A. & Vale, W. Brain Res. 239, 463–477 (1982).
Srikant, C. V. & Patel, Y. C. Proc. natn. Acad. Sci. U.S.A. 78, 3930–3934 (1981).
Tapia-Arancibia, L., Epelbaum, J., Enjalbert, A. & Kordon, C. Eur. J. Pharmac. 71, 523–525 (1981).
Epelbaum, J., Tapia-Arancibia, L., Kordon, C. & Enjalbert, A. J. Neurochem. 38, 1515–1523 (1982).
Reubi, J.-C., Perrin, M., Rivier, J. & Vale, W. Life Sci. 28, 2191–2198 (1981).
Benoit, R. et al. Proc. natn. Acad. Sci. U.S.A. 79, 917–921 (1982).
Benoit, R., Morrison, J., Alford, B. & Bakhit, C. Endocrinology 110, A913 (1982).
Weakly, J. N. J. Physiol., Lond. 234, 597–612 (1973).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bakhit, C., Benoit, R. & Bloom, F. Release of somatostatin-28(1–12) from rat hypothalamus in vitro. Nature 301, 524–526 (1983). https://doi.org/10.1038/301524a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/301524a0
This article is cited by
-
Molecular biology of the regulation of hypothalamic hormones
Journal of Endocrinological Investigation (1993)
-
HPLC analysis of somatostatin related peptides in putamen of Huntington's Disease patients
Journal of Neural Transmission (1987)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.