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T-cell hybridomas which produce B lymphocyte replication factors only

Nature volume 300, pages 355–357 (1982)Cite this article

Abstract

B lymphocytes stimulated with BRMF (B-cell replication and maturation factor) expand clonally and mature into immunoglobulin (Ig)-secreting cells. BRMF may be a single or separate factors (BRF, BMF), capable of inducing replication and maturation in B cells1–3. Experimental evidence from others4,5 suggests that BRF and BMF may be distinct molecular entities. We now find that some hybrids of concanavalin A (Con A)-activated splenic T cells and the thymoma BW5147 produce BRF on stimulation with Con A, but do not produce BMF, T-cell growth factor (TCGF), T-cell replacing factor (TRF) or factors stimulating myeloid or erythroid colonies (CSF). Thus BRF and BMF are distinct activities. BRF produced by the T-cell hybridoma A32-26 appears in two major forms with molecular weights of 15,000–20,000 and 80,000–90,000.

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Authors and Affiliations

  1. Basel Institute for Immunology, CH-4005, Basel, Switzerland

    Waldemar Lernhardt, Catherine Corbel & Fritz Melchers

  2. Molecular Biology Institute, University of California at Los Angeles, Los Angeles, California, 90024, USA

    Waldemar Lernhardt & Randolph Wall

Authors
  1. Waldemar Lernhardt
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  2. Catherine Corbel
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  3. Randolph Wall
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  4. Fritz Melchers
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Lernhardt, W., Corbel, C., Wall, R. et al. T-cell hybridomas which produce B lymphocyte replication factors only. Nature 300, 355–357 (1982). https://doi.org/10.1038/300355a0

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