Abstract
Genes lacking the intervening sequences that are present in otherwise apparently homologous genes have been found in some highly oncogenic retroviruses (some viral oncogenes) and in the normal cell genome (cDNA genes, pseudogenes or processed genes)1–10. Such cDNA genes might have arisen by reverse transcription of the spliced (intervening sequences removed) RNA transcript of the homologous genes. Another possible way for genes with intervening sequences to lose such sequences would be during replication in a retrovirus vector. To test this hypothesis we inserted genomic mouse α-globin DNA containing two intervening sequences11 into the DNA of an infectious retrovirus vector12 and report here that the intervening sequences were removed from DNA of progeny virus during virus replication.
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Shimotohno, K., Temin, H. Loss of intervening sequences in genomic mouse α-globin DNA inserted in an infectious retrovirus vector. Nature 299, 265–268 (1982). https://doi.org/10.1038/299265a0
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DOI: https://doi.org/10.1038/299265a0
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