Abstract
The macrophage is a highly phagocytic cell which has been widely used in attempts to dissect the mechanisms of endocytosis1. In immune phagocytosis specific interactions occur between ligands on the presenting particle and Fc or C3b specific receptors expressed on the plasma membrane2,3, but macrophages also avidly ingest particles such as polystyrene latex by an immunologically nonspecific mechanism. This process is guided by sequential ligand–receptor interactions4,5. Biochemical studies indicate that actin, myosin and several regulatory components distinct from the troponin system of skeletal muscle, take part in phagocytosis by macrophages and polymorphonuclear leukocytes (PMN)6 and indirect immunofluorescence confirms the presence of these components at sites of phagocytic activity7. However, comparatively little is known about coupling between receptor binding and assembly of the contractile system, although electrical changes8 and the flux of divalent cations9 have been implicated. Here we report a monoclonal antibody that detects an intracellular protein from macrophages which binds Ca2+ in non-phagocytosing cells, and becomes phosphorylated as a result of phagocytosis. It might therefore take part in signal transduction during this process.
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References
Silverstein, S. C., Steinman, R. M. & Cohn Z. A. A. Rev. Biochem. 46, 669–722 (1977).
Unkeless, J. C. J. exp. Med. 145, 931–947 (1977).
Bianco, C., Griffin, F. M. & Silverstein, S. C. J. exp. Med. 141, 1278–1290 (1975).
Griffin, F. M. & Silverstein, S. C. J. exp. Med. 139, 323–336 (1974).
Griffin, F. M., Griffin, J. A. & Silverstein, S. C. J. exp. Med. 144, 788–809 (1976).
Stossel, T. P., Hartwig, J. H., Yin, H. L. & Stendahl, O. Biochem. Soc. Symp. 45, 51–63 (1981).
Stendahl, O., Hartwig, J. H., Brotschi, E. A. & Stossel, T. P. J. Cell Biol. 84, 215–224 (1980).
Kouri, J., Noa, M., Diaz, B. & Nimbo, E. Nature 283, 868–869 (1980).
Hoffstein, S. T. J. Immun. 123, 1395–1402 (1979).
Wetzel, M. G. & Korn, E. D. J. Cell Biol. 43, 90–104 (1969).
Galfre, G., Milstein, C. & Wright, B. Nature 277, 131 (1979).
Austyn, J. M. & Gordon, S. Eur. J. Immun. 11 (1981).
Klee, C. B., Crouch, T. H. & Richman, R. G. A. Rev. Biochem. 49, 489–515 (1980).
Kendrick-Jones, J. & Scholey, J. M. J. Muscle Res. Cell Motility 2, 347–372 (1981).
Watterson, D. M., Iverson, D. B. & van Eldik, L. H. Biochemistry 19, 5762–5768 (1980).
Scholey, J. M., Taylor, K. A. & Kendrick-Jones, J. Nature 287, 233–235 (1980).
Stossel, T. P. J. Cell Biol. 58, 346–356 (1973).
Endo, M. Physiol. Rev. 57, 71–108 (1977).
Schneider, C., Zanetti, M. & Romeo, D. FEBS Lett. 127, 4–8 (1981).
Morris, R. J. & Williams, A. F. Eur. J. Immun. 5, 274 (1975).
Hunt, S. V. & Fowler, M. H. Cell Tissue Kinetics 14, 445–464 (1981).
Laemmli, U. K. Nature 227, 680 (1970).
Bonner, W. M. & Laskey, R. A. Eur. J. Biochem. 46, 83 (1974).
Bowen, B., Steinberg, J., Laemmli, U. K. & Weintraub, H. Nucleic Acids Res. 8, 1–20 (1980).
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Vaux, D., Gordon, S. Monoclonal antibody defines a macrophage intracellular Ca2+-binding protein which is phosphorylated by phagocytosis. Nature 299, 70–72 (1982). https://doi.org/10.1038/299070a0
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DOI: https://doi.org/10.1038/299070a0
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