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Left-handed DNA in restriction fragments and a recombinant plasmid

Nature volume 290, pages 672–677 (1981)Cite this article

Abstract

Circular dichroism and 31P-NMR on synthetic oligomers of (dC-dG) inserted within DNA restriction fragments indicate that the right-handed B-structure can exist in close proximity to the left-handed Z-structure. Also, this salt-induced transition to Z-form in a small (dC-dG) segment (1.3%) of a recombinant plasmid markedly influenced the supercoil of the plasmid. These observations have implications for the postulated role of naturally occurring related simple sequences in the regulation of gene activity.

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Authors and Affiliations

  1. University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, Wisconsin, 53706, USA

    Jan Kłysik, Steven M. Stirdivant, Jacquelynn E. Larson, Phillip A. Hart & Robert D. Wells

  2. School of Pharmacy, University of Wisconsin, Madison, Wisconsin, 53706, USA

    Jan Kłysik, Steven M. Stirdivant, Jacquelynn E. Larson, Phillip A. Hart & Robert D. Wells

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  1. Jan Kłysik
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  4. Phillip A. Hart
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  5. Robert D. Wells
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Kłysik, J., Stirdivant, S., Larson, J. et al. Left-handed DNA in restriction fragments and a recombinant plasmid. Nature 290, 672–677 (1981). https://doi.org/10.1038/290672a0

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