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Interferon treatment inhibits glycosylation of a viral protein

Nature volume 287, pages 454–456 (1980)Cite this article

Abstract

Earlier, we reported1 a 30–200-fold reduction in the yield of infectious vesicular stomatitis virus (VSV) released from L cells treated with 10–30 reference units ml−1 of interferon (IFN); however, in these cultures virus particle production, as measured by VSV particle-associated viral RNA, virus nucleocapsid protein and viral transcriptase, was inhibited less than 10-fold. There was biochemical and morphological evidence of a significant reduction in glycoprotein (G) and membrane protein (M) of VSV particles released from IFN-treated cells2,3. We compare here the effects of tunicamycin (TM) and IFN in L cells. Treatment with TM or IFN reduced the production of infectious VSV particles, decreased the amount of G and M proteins in VSV released from treated cells, and inhibited an early step in the formation of asparagine-linked oligosaccharide chains, the incorporation by membrane preparations from treated cells of N-acetylglucosamine into glycolipids with the properties of dolichol derivatives.

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Authors and Affiliations

  1. Laboratory of Experimental Pathology, NIAMDD, National Institutes of Health, Bethesda, Maryland, 20205

    Radha K. Maheshwari & Robert M. Friedman

  2. Department of Biological Chemistry, University of Maryland School of Medicine, Baltimore, Maryland, 21201

    Dipak K. Banerjee & Charles J. Waechter

  3. Howard University Cancer Research Center, Washington DC, 20060

    Kenneth Olden

Authors
  1. Radha K. Maheshwari
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  2. Dipak K. Banerjee
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  3. Charles J. Waechter
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  4. Kenneth Olden
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  5. Robert M. Friedman
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Maheshwari, R., Banerjee, D., Waechter, C. et al. Interferon treatment inhibits glycosylation of a viral protein. Nature 287, 454–456 (1980). https://doi.org/10.1038/287454a0

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