Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Immunochemical identification of an actin-like protein from soybean seedlings

Nature volume 285, pages 171–172 (1980)Cite this article

Abstract

Microfilaments are recognised as ubiquitous cytoskeletal and contractile elements found in virtually all types of cells. Indeed, the major protein component of these fibrillar structures, actin, has been isolated from a variety of cells1–5. A number of laboratories have recently reported ultrastructural observations of microfilaments in plant cells such as Avena sativa, Nicotiana tabacum, Nitella flexilis, Amaryllis belladonna, Haemanthus katherina, Mougeotia, Mimosa and Chara6–13. Preliminary studies on the identification of the actin subunit of these structures have also been reported for Nitella, Phaseolus vulgaris and Lycopesicon esculentum14–16. More detailed studies have been made on actin isolated from the cellular slime mould, Dictyostelium discoideum17,18. We report here the isolation, from extracts of soybean (Glycine max) seedlings, of a protein that binds rabbit antibodies directed against calf thymus actin. The results suggest the presence of an actin-like polypeptide chain in soybean cells.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Pollard, T. D. & Weihing, R. R. Crit. Rev. Biochem. 2, 1–65 (1974).

    Article  CAS  Google Scholar 

  2. Hepler, P. K. & Palevitz, B. A. A. Rev. Pl. Physiol. 25, 309–362 (1974).

    Article  CAS  Google Scholar 

  3. Clarke, M. & Spudich, J. A. A. Rev. Biochem. 46, 797–822 (1977).

    Article  CAS  Google Scholar 

  4. Korn, E. D. Proc. natn. Acad. Sci. U.S.A. 75, 588–599 (1978).

    Article  ADS  CAS  Google Scholar 

  5. Goldman, R. D., Milsted, A., Schloss, J. A., Starger, J. & Yerna, M. J. A. Rev. Physiol. 41, 703–722 (1979).

    Article  CAS  Google Scholar 

  6. O'Brien, T. P. & Thimann, K. V. Proc. natn. Acad. Sci. U.S.A. 56, 888–894 (1966).

    Article  ADS  CAS  Google Scholar 

  7. Parthasarathy, M. V. & Muhlethaler, K. J. Ultrastruct. Res. 38, 46–62 (1972).

    Article  CAS  Google Scholar 

  8. Palevitz, B. A., Ash, J. F. & Hepler, P. K. Proc. natn. Acad. Sci. U.S.A. 71, 363–366 (1974).

    Article  ADS  CAS  Google Scholar 

  9. Condeelis, J. S. Expl Cell Res. 88, 435–439 (1974).

    Article  CAS  Google Scholar 

  10. Forer, A. & Jackson, W. T. Cytobiologie 10, 217–226 (1975).

    CAS  Google Scholar 

  11. Marchant, H. J. Planta 131, 119–120 (1976).

    Article  CAS  PubMed  Google Scholar 

  12. Arraes-Hermans, M. A. & Cruz, W. B. Revue Microbiol. 3, 8–9 (1976).

    Google Scholar 

  13. Williamson, R. E. Nature 248, 801–802 (1974).

    Article  ADS  CAS  PubMed  Google Scholar 

  14. Kato, T. & Tonomura, Y. J. Biochem. 82, 777–782 (1977).

    Article  CAS  PubMed  Google Scholar 

  15. Jackson, W. T. & Doyle, B. G. J. Cell Biol. 75, 268a (1977).

    Article  Google Scholar 

  16. Vahey, M., Trantwein, R. & Scordillis, S. P. Biophys. J. 21, 23a (1978).

    Google Scholar 

  17. Wolley, D. E. Archs Biochem. Biophys. 150, 579–530 (1972).

    Article  Google Scholar 

  18. Uyemura, D. G., Brown, S. S. & Spudich, J. A. J. biol. Chem. 253, 9088–9096 (1978).

    CAS  PubMed  Google Scholar 

  19. Williams, C. A. & Chase, M. W. (eds) in Methods in Immunology and Immunochemistry Vol. 1 (Academic, New York, 1967).

  20. Greenwood, F. C., Hunter, W. M. & Glover, J. S. Biochem. J. 89, 114–123 (1963).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Vietta, E. S., Baur, S. & Uhr, J. W. J. exp. Med. 134, 242–264 (1971).

    Article  Google Scholar 

  22. Weber, K. & Osborn, M. J. biol. Chem. 244, 4406–4412 (1969).

    CAS  PubMed  Google Scholar 

  23. Gordon, D. J., Eisenberg, E. & Korn, E. D. J. biol. Chem. 251, 1778–1786 (1976).

    Google Scholar 

  24. Cuatercasas, P. J. biol. Chem. 245, 3059–3065 (1970).

    Google Scholar 

  25. Neimark, H. C. Proc. natn. Acad. Sci. U.S.A. 74, 4041–4045 (1977).

    Article  ADS  CAS  Google Scholar 

  26. Piperno, G. & Luck, D. J. L. J. biol. Chem. 254, 2187–2190 (1979).

    CAS  PubMed  Google Scholar 

  27. LeStourgeon, W. M., Forer, A., Yang, Y-Z., Betram, J. S. & Rusch, H. P. Biochim. biophys. Acta 379, 529–552 (1975).

    Article  CAS  PubMed  Google Scholar 

  28. Jockusch, B. M., Becker, M., Hindennach, I. & Jockusch, H. Expl Cell Res. 89, 241–246 (1974).

    Article  CAS  Google Scholar 

  29. Weihing, R. R. & Korn, E. D. Biochemistry 1, 590–600 (1971).

    Article  Google Scholar 

  30. Pollard, T. D. J. Cell Biol. 68, 579–601 (1976).

    Article  CAS  PubMed  Google Scholar 

  31. Bejanian, M. V., Surguchov, A. P. & Smirnov, V. W. FEBS Lett. 102, 55–58 (1979).

    Article  PubMed  Google Scholar 

  32. O'Farrell, P. H. J. biol. Chem. 250, 4007–4021 (1975).

    CAS  PubMed  Google Scholar 

  33. Barany, M., Barany, K. & Guba, E. Nature 179, 818–819 (1957).

    Article  ADS  CAS  PubMed  Google Scholar 

  34. Spudich, J. A. & Watt, S. J. biol. Chem. 246, 4866–4871 (1971).

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, Michigan State University, East Lansing, Michigan, 48824

    Thomas N. Metcalf III, Leslie J. Szabo, Karel R. Schubert & John L. Wang

Authors
  1. Thomas N. Metcalf III
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Leslie J. Szabo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Karel R. Schubert
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. John L. Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Metcalf, T., Szabo, L., Schubert, K. et al. Immunochemical identification of an actin-like protein from soybean seedlings. Nature 285, 171–172 (1980). https://doi.org/10.1038/285171a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/285171a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing