Abstract
HUMAN fibroblast interferon is a single monomeric gly-coprotein1, and presumably a single structural gene codes for the human interferon polypeptide. It was originally reported that chromosomes 2 and 5 were necessary together for human interferon production2. More recent evidence suggests that both chromosomes 2 and 5 carry separate human interferon genes3,4. However, we have found, in two separate series of human–mouse somatic cell hybrid cell lines and their diphtheria toxin-resistant subclones5,6, that human interferon production segregated from chromosome 5 and, furthermore, did not correlate with the presence of chromosomes 2 and 5 together7. Furthermore, using 29 hybrid clones (13 independent primary hybrids and 16 subclones) we could exclude chromosomes 1–8, 10–22 and X as candidates for the localisation of a single gene coding for human interferon on the basis of discordance of more than three clones for the segregation of human interferon production and the presence of a particular chromosome. However, there was a good correlation between the production of human interferon and the presence of chromosome 9. Here we demonstrate this directly.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Knight, E. Jr Proc. natn. Acad. Sci. U.S.A. 73, 520–523 (1976).
Tan, Y. H., Creagan, R. P. & Ruddle, F. H. Proc. natn. Acad. Sci. U.S.A. 71, 2251–2255 (1974).
Tan, Y. H. in Interferons and their Actions (ed. Stewart, W. E. II) Ch. 5 (CRC Press, Cleveland, Ohio, 1977).
Slatz, D. L. & Ruddle, F. H. Cell 16, 171–180 (1979).
Creagan, R. P., Chen, S. & Ruddle, F. H. Proc. natn. Acad. Sci. U.S.A. 72, 2237–2241 (1975).
Swallow, D. M., Solomon, E. & Pajunen, L. Cytogenet. Cell Genet. 18, 136–148 (1977).
Meager, A. et al. J. gen. Virol. (in the press).
Pearson, P. L., Sanger, R. & Brown, J. A. Cytogenet. Cell Genet. 13, 190–195 (1974).
Shows, T. B. & Brown, J. A. Proc. natn. Acad. Sci. U.S.A. 72, 2125–2129 (1975).
Cox, R. P., Krauss, M. R., Balis, M. E. & Dancis, J. Expl. Cell. Res. 24, 217–254 (1972).
Jonasson, J., Povey, S. & Harris, H. J. Cell Sci. 24, 217–254 (1977).
Davidson, R. L. & Gerald, P. S. Somatic Cell Genet. 2, 165–176 (1976).
Mankovitz, R., Buchwald, M. & Baker, R. M. Cell 3, 221–226 (1974).
Atkins, G. J., Johnston, M. D., Westmacott, L. M. & Burke, D. C. J. gen. Virol. 25, 381–390 (1974).
Bishun, N. P., Williams, D. C. & Doyle, P. in Laboratory Manual of Cell Biology (eds Hall, D. & Hawkins, S.) 76–78 (English Universities Press, London, 1975).
Harris, H. & Hopkinson, D. A. Handbook of Enzyme Electrophoresis in Human Genetics (North-Holland, Amsterdam, 1976).
Harris, H. & Hopkinson, D. A. Handbook of Enzyme Electrophoresis in Human Genetics, Suppl. (North-Holland, Amsterdam, 1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
MEAGER, A., GRAVES, H., BURKE, D. et al. Involvement of a gene on chromosome 9 in human fibroblast Interferon production. Nature 280, 493–495 (1979). https://doi.org/10.1038/280493a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/280493a0
This article is cited by
-
Comparative map for mice and humans
Mammalian Genome (1992)
-
Induction of interferon in hybrid clones: Vero 153-mouse myeloma and Vero 153-human lymphocyte cells
Somatic Cell Genetics (1983)
-
Differences between human ? (leukocyte) and ? (fibroblast) interferons
Archives of Virology (1981)
-
Heterogeneity of poly(I).poly(C)-induced human fibroblast interferon mRNA species
Nature (1980)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.