Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

DNA Sequence of the mini-insertion IS2–6 and its relation to the sequence of IS2

Abstract

In polar orientation IS2 abolishes galactose operon expression. Operon activity is restored by a 108 base pair mini-insertion within IS2 called IS2–6. The DNA sequences of the gal operon -IS2 junction, the parental IS2 region undergoing sequence rearrangements and IS2–6 itself are reported. IS2–6 is composed of sequence intervals present in both strands of IS2.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Saedler, H. & Heiss, B. Molec. gen. Genet. 22, 267–277, (1973).

    Article  Google Scholar 

  2. Starlinger, P. & Saedler, H. in Curr. Topics Microbiol. Immun. 75, 111 (1975).

    Google Scholar 

  3. Saedler, H., Reif, H. J., Hu, S. & Davidson, N. Molec. gen. Genet. 132, 265–289 (1974).

    Article  CAS  Google Scholar 

  4. Pilacinsky, W. et al. Gene 2, 61–74 (1977).

    Article  Google Scholar 

  5. Ghosal, D. & Saedler, H. Molec. gen. Genet. 158, 123–128 (1977).

    Article  CAS  Google Scholar 

  6. Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).

    Article  ADS  CAS  Google Scholar 

  7. Musso, R. E. & Rosenberg, M. in DNA Insertion Elements, Plasmids, and Episomes (eds Bukhari, A. I., Shapiro, J. A. & Adhya, S. L.) 597–600 (Cold Spring Harbor, 1977).

    Google Scholar 

  8. Streeck, R. E. & Hobom, G. Eur. J. Biochem. 57, 595–606 (1975).

    Article  CAS  Google Scholar 

  9. Thuring, R. W. I., Sanders, I. P. M. & Borst, P. Analyt. Biochem. 66, 213–221 (1975).

    Article  CAS  Google Scholar 

  10. Brack, Ch., Eberle, H., Bickle, T. A. & Yuan, R. J. molec. Biol. 10, 305–309 (1976).

    Article  Google Scholar 

  11. Musso, R., Di Lauro, R., Rosenberg, M. & de Crombrugghe, B. Proc. natn. Acad. Sci. U.S.A. 74, 106–110 (1977).

    Article  ADS  CAS  Google Scholar 

  12. Schlesinger, D. H., Niall, H. D. & Wilson, D. Biochem. biophys. Res. Comm. 61, 282–289 (1974).

    Article  CAS  Google Scholar 

  13. Ghosal, D. & Saedler, H. Cold Spring Harb. Symp. quant. Biol. 43, (in the press).

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ghosal, D., Saedler, H. DNA Sequence of the mini-insertion IS2–6 and its relation to the sequence of IS2. Nature 275, 611–617 (1978). https://doi.org/10.1038/275611a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/275611a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing