Abstract
EXHAUSTIVE T1 ribonuclease digestion of the RNA of tobacco mosaic virus (TMV) liberates an unusually large T1 oligonucleotide (∼72 nucleotides) called Ω (refs 1–4). Analysis of partial T1 ribonuclease digestion products of uniformly 32P-labelled TMV RNA shows that Ω is no more than 200 nucleotides from the 5′;-end of the chain. We have used polynucleotide kinase to fix a radioactive phosphate to the 5′-end of both Ω and intact TMV RNA (after removal of the pppm7G ‘cap’) and determined the sequence of the first 15–16 nucleotides at the 5′ terminus of each by two-dimensional homochromatography of partial P1 nuclease digests. The results show that Ω begins only one nucleotide away from the 5′-end of the RNA chain, the terminal sequence being m7GpppGΩ… Consequently, positions 2–72 of the TMV RNA molecule are devoid of G (guanosine residues).
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RICHARDS, K., JONARD, G., GUILLEY, H. et al. Leader sequence of 71 nucleotides devoid of G in tobacco mosaic virus RNA. Nature 267, 548–550 (1977). https://doi.org/10.1038/267548a0
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DOI: https://doi.org/10.1038/267548a0
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