Abstract
SPLEENS of normal mice contain large numbers of cells which produce antibody against antigenic determinants on their own erythrocytes1,2. To demonstrate this autoimmune response, the mouse erythrocytes (MRBC) must be treated with proteolytic enzymes, such as trypsin or bromelain, in order to reveal hidden autoantigen(s)2. Treatment of mice with anti-lymphocyte serum (ALS) causes a 20-fold rise in the number of cells producing antibody to bromelain-treated mouse erythrocytes (BrMRBC) (ref. 2). Based on this observation, Cunningham has proposed that the number of cells making anti-BrMRBC antibody is controlled by an active, ongoing, T-cell induced suppression3. We report here that the culturing of normal mouse spleen cells in vitro not only allows the development of cells producing antibody to BrMRBCs, but also T cells which mediate, on transfer, delayed-type hypersensitivity (DTH).
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Cunningham, A. J. Nature 252, 749–751 (1974).
Cunningham, A. J. Nature 254, 143–144 (1975).
Cunningham, A. J. Transplantatn Rev. 31, 23–43 (1976).
Marbrook, J. Lancet ii, 1279–1281 (1967).
Ramshaw, I. A., Bretscher, P. A. & Parish, C. R. Eur. J. Immun. 6, 674–679 (1976).
Parish, C. R., Kirov, S. M., Bowern, N. & Blanden, R. V. Eur. J. Immun. 4, 808–815 (1974).
Pages, J. & Bussard, A. E., Nature 257, 316–317 (1975).
Ramshaw, I. A., Bretscher, P. A. & Parish, C. R. Eur. J. Immun. (in the press).
Ramshaw, I. A., McKensie, I. F. C., Bretscher, P. A. & Parish, C. R. Cell. Immun. (in the press).
Lance, E. M., Medawar, P. B. & Taub, R. N. Adv. Immun. 17, 1–92 (1973).
Reisner, E. E. & Amos, D. B. Transplantation 14, 455–461 (1972).
Rogentine, G. N. & Plocinik, B. A. J. Immun. 113, 848–858 (1974).
Rosenberg, S. A. & Rogentine, G. N. Nature new Biol. 239, 203–204 (1972).
Herberman, R. B. & Aoki, T. J. exp. Med. 136, 94–111 (1972).
Martin, S. T. & Martin, W. J. Int. J. Cancer 15, 658–664 (1975).
Gomard, E., Leclerc, J. C. & Levy, J. P. Nature 250, 671–673 (1974).
Herberman, R. B., Nunn, M. B. & Lourin, D. H. Int. J. Cancer 16, 216–229 (1975).
Davidson, W. F. & Parish, C. R. J. immun. Meth. 7, 291–299 (1975).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
RAMSHAW, I., EIDINGER, D. T-cell mediated immunity towards antigen(s) on isologous erythrocytes. Nature 267, 441–442 (1977). https://doi.org/10.1038/267441a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/267441a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
