Abstract
THE serological detection of the H–Y (male) antigen in the mouse has been demonstrated1 using the cytotoxicity test against mouse spermatoza, and that work has been confirmed2 by the demonstration of H–Y on male (not female) mouse epidermal cells. There are, however, technical difficulties associated with both assays and these are hard to overcome. We report here a simplified method for the serological detection of H–Y antigen on mouse lymphocytes using Staphylococcus aureus (SA) as a marker. We do this in the interests of other investigators who may wish to investigate the H–Y system by a relatively simple method.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Goldberg, E. H., Boyse, E. A., Bennett, D., Scheid, M. & Carswell, E. A. Nature 232, 478–480 (1971).
Scheid, M., Boyse, E. A., Carswell, E. A. & Old, L. J. J. exp. Med. 135, 938–955 (1972).
Kronvall, G., Seal, U. S., Finstad, J. & Williams, R. C. Jr., J. Immun. 104, 140–147 (1970).
Sjoquist, J., Movitz, J., Johansson, I. B. & Hjelm, H. Eur. J. Biochem. 30, 190–194 (1972).
Kearney, R., Chia, E. & Basten, A. J. Immun. 114, 1143–1146 (1975).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
TOKUDA, S., ARRINGTON, T., GOLDBERG, E. et al. Simpler technique for serological detection of H–Y antigen on mouse lymphocytes. Nature 267, 433–434 (1977). https://doi.org/10.1038/267433a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/267433a0
This article is cited by
-
Comments on the paper by M. T. Zenzes and T. E. Reed hum genet 66:103?109 (1984)
Human Genetics (1984)
-
Identification and function of serologically detectable H-Y antigen
Human Genetics (1982)
-
H-Y antigen in transsexuality, and how to explain testis differentiation in H-Y antigen-negative males and ovary differentiation in H-Y antigen-positive females
Human Genetics (1980)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
