Abstract
The structure of a specific acyl-enzyme intermediate in the elastase-catalysed hydrolysis of N-carbobenzoxy-L-alanyl-p-nitrophenol ester has been determined by X-ray diffraction at 3.5 Å resolution. The acyl-enzyme was stabilised by cooling the crystal to −55°C during substrate addition and data collection.
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Alber, T., Petsko, G. & Tsernoglou, D. Crystal structure of elastase–substrate complex at −55 °C. Nature 263, 297–300 (1976). https://doi.org/10.1038/263297a0
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DOI: https://doi.org/10.1038/263297a0
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