Abstract
WE have reported the three-dimensional structure of yeast tRNAPhe, determined by fitting a skeletal wire model to an electron density map at a resolution of 2.5 Å, phased by the method of isomorphous replacement1. Atomic coordinates have been published for the unrefined structure2. We are in the process of refining our structure using difference Fourier techniques together with the real-space refinement procedure of Diamond3. The crystallographic residual (R) at 2.5 Å is 0.31, a value comparable to that found for proteins at a similar stage.
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References
Ladner, J. E., et al., Proc. natn. Acad. Sci. U.S.A., 72, 4414–4418 (1975).
Ladner, J. E., Jack, A., Robertus, J. D., Brown, R. S., and Rhodes, D., Nucleic Acid Res., 2, 1629–1637 (1975).
Diamond, R., Acta Crystallogr., A 27, 436–452 (1971).
Arnott, S., and Hukins, D. W. L., Nature, 224, 886–888 (1969).
Sundaralingam, M., in Conformation of Biological Molecules and Polymers, 417–455 (Israel Academy of Sciences and Humanities, Jerusalem, 1973).
Kim, S.-H., Berman, H. M., Seeman, N. C., and Newton, M. D., Acta Crystallogr., B 29, 703–710 (1973).
Yathindra, N., and Sundaralingam, M., Proc. natn. Acad. Sci. U.S.A., 71, 3325–3328 (1974).
Crick, F. H. C., and Klug, A., Nature, 255, 530–533 (1975).
Viswamitra, M. A., Seshadri, T. P., and Post, M. L., Nature, 258, 540–541 (1975).
Voet, D., and Rich, A., Proc. natn. Acad. Sci. U.S.A., 68, 1151–1156 (1971).
Quigley, G. Q., Seeman, N. C., Wang, A. H.-J., Suddath, F. L., and Rich, A., Nucleic Acids Res., 2, 2329–2341 (1975).
Sussman, J. L., and Kim, S.-H., Biochem. biophys. Res. Commun., 68, 89–95 (1976).
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JACK, A., KLUG, A. & LADNER, J. “Non-rigid” nucleotides in tRNA: a new correlation in the conformation of a ribose. Nature 261, 250–251 (1976). https://doi.org/10.1038/261250a0
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DOI: https://doi.org/10.1038/261250a0
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