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Isolation and N-terminal amino acid sequence of membrane-bound human HLA-A and HLA-B antigens

Nature volume 261, pages 200–205 (1976)Cite this article

Abstract

Membrane-bound HLA-A and HLA-B antigens have been extensively purified in good yield. The sequences of the N-terminal 16 amino acids have been determined using about 1 nmol of protein eluted from polyacrylamide gel after electrophoresis in sodium dodecyl sulphate.

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Author information

Authors and Affiliations

  1. Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK

    John Bridgen

  2. National Institute for Medical Research, Mill Hill, London, NW7 1AA, UK

    David Snary & Michael J. Crumpton

  3. Genetics Laboratory, Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UK

    Colin Barnstable, Peter Goodfellow & Walter F. Bodmer

Authors
  1. John Bridgen
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  2. David Snary
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  3. Michael J. Crumpton
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  4. Colin Barnstable
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  5. Peter Goodfellow
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  6. Walter F. Bodmer
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Reprint requests to M. J. Crumpton at the above address.

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Bridgen, J., Snary, D., Crumpton, M. et al. Isolation and N-terminal amino acid sequence of membrane-bound human HLA-A and HLA-B antigens. Nature 261, 200–205 (1976). https://doi.org/10.1038/261200a0

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  • DOI: https://doi.org/10.1038/261200a0

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