Abstract
IT has been adequately demonstrated that BUdR is mutagenic in bacteriophage1,2,3 when incorporated in place of thymidine in DNA, whereas a clear demonstration that it is mutagenic in eukaryotic cells is still lacking. We have described the selection of two variant hamster cell lines that survive with extensive levels of BUdR substitution in DNA4,5 and that have been maintained in continuous cultivation for more than 300 generations without loss of viability. We have found no evidence of alterations in the nucleotide ratio of nuclear DNA consistent with the accumulation of BUdR-induced base transition on long term cultivation of the cells, and analysis of rRNA made in the substituted cells offers no evidence that BUdR causes base transitions within the genome or transcriptional errors which result in the accumulation of faulty RNA transcripts6.
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BICK, M., SOFFER, M. Altered glucose-6-phosphate dehydrogenase in bromodeoxyuridine-substituted cells. Nature 260, 788–791 (1976). https://doi.org/10.1038/260788a0
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DOI: https://doi.org/10.1038/260788a0
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