Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Mechanism localisation and control of restriction cleavage of phage T4 and λ chromosomes in vivo

Nature volume 260, pages 406–410 (1976)Cite this article

Abstract

The primary action of restriction endonuclease, cleaving infecting DNA, has been demonstrated in vivo. This primary cleavage is followed rapidly by hydrolysis of the cleaved DNA at its newly exposed termini. Infecting viruses can inactivate cytoplasmic and membrane restriction endonucleases to prevent cleavage of unmodified DNA replicas.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Science, 187, 931–935 (1975).

    Article  ADS  PubMed  Google Scholar 

  2. Nathans, D., and Smith, H. O., A. Rev. Biochem., 44, 273–293 (1975).

    Article  CAS  Google Scholar 

  3. Meselson, M., Yuan, R., and Heywood, J., A. Rev. Biochem., 41, 447–446 (1972).

  4. Yuan, R., Bickle, T. A., Ebbers, W., and Brack, C., Nature, 256, 556–560 (1975).

    Article  ADS  CAS  PubMed  Google Scholar 

  5. Greene, P. J., et al., J. molec. Biol., 99, 237–261 (1975).

    Article  CAS  PubMed  Google Scholar 

  6. Polisky, B., et al., Proc. natn. Acad. Sci. U.S.A., 72, 3301–3314 (1975).

    ADS  Google Scholar 

  7. Schlagman, S., Hattman, S., May, M. S., and Berger, L., J. Bact. (in the press).

  8. Studier, F. W., J. molec. Biol., 94, 283–295 (1975).

    Article  CAS  PubMed  Google Scholar 

  9. Meselson, M., and Yuan, R., Nature, 217, 1110–1114 (1968).

    Article  ADS  CAS  PubMed  Google Scholar 

  10. Smith, H. O., and Wilcox, K. W., J. molec. Biol., 51, 379–391 (1970).

    Article  CAS  PubMed  Google Scholar 

  11. Horiuchi, K., and Zinder, N. D., Proc. natn. Acad. Sci. U.S.A., 69, 3220–3224 (1972).

    Article  ADS  CAS  Google Scholar 

  12. Murray, N. E., Batten, B. L., and Murray, K., J. molec. Biol., 81, 395–407 (1973).

    Article  CAS  PubMed  Google Scholar 

  13. Horiuchi, K., Vovis, G. F., and Zinder, N. D., J. biol. Chem., 249, 543–552 (1974).

    CAS  PubMed  Google Scholar 

  14. Horiuchi, K., and Zinder, N. D., Proc. natn. Acad. Sci. U.S.A., 72, 2555–2558 (1975).

    Article  ADS  CAS  Google Scholar 

  15. Arber, W., J. molec. Biol., 11, 247–256 (1965).

    Article  CAS  PubMed  Google Scholar 

  16. Klein, A., and Sauerbier, W., Biochem. biophys. Res. Commun., 18, 440–445 (1965).

    Article  CAS  PubMed  Google Scholar 

  17. Hattman, S., and Fukasawa, T., Proc. natn. Acad. Sci. U.S.A., 50, 297–300 (1963).

    Article  ADS  CAS  Google Scholar 

  18. Shedlovsky, A., and Brenner, S., Proc. natn. Acad. Sci. U.S.A., 50, 300–306 (1963).

    Article  ADS  CAS  Google Scholar 

  19. Symonds, N., Stacey, K. A., Glover, S. W., Schell, J., and Silver, S., Biochem. biophys. Res. Commun., 12, 220–222 (1963).

    Article  CAS  PubMed  Google Scholar 

  20. Hedgpeth, J., Goodman, H. M., and Boyer, H. W., Proc. natn. Acad. Sci. U.S.A., 69, 3448–3452 (1972).

    Article  ADS  CAS  Google Scholar 

  21. Dussoix, D., and Arber, W., J. molec. Biol., 5, 37–49 (1962).

    Article  CAS  PubMed  Google Scholar 

  22. Fukasawa, T., J. molec. Biol., 9, 525–536 (1964).

    Article  CAS  PubMed  Google Scholar 

  23. Hattman, S., Virology, 24, 333–348 (1964).

    Article  CAS  PubMed  Google Scholar 

  24. Paigen, K., and Weinfeld, H., Virology, 19, 565–572 (1963).

    Article  CAS  PubMed  Google Scholar 

  25. Glover, S. W., Uptake of Informative Molecules by Living Cells (edit by Ledouz, L.), 185–211 (North-Holland, Amsterdam, 1972).

    Google Scholar 

  26. Arber, W., Progress in Nucleic Acid Research and Molecular Biology, 14 (edit. by Cohn, W. E,), 1–34 (Academic, New York, 1974).

    Google Scholar 

  27. Simmon, V. F., and Lederberg, S., J. Bact., 112, 161–169 (1972).

    CAS  PubMed  PubMed Central  Google Scholar 

  28. Barbour, S. D., and Clark, A. J., Proc. natn. Acad. Sci. U.S.A., 65, 955–961 (1970).

    Article  ADS  CAS  Google Scholar 

  29. Silverstein, J., and Goldberg, E. B., Virology (in the press).

  30. Silverstein, J., and Goldberg, E. B., Virology (in the press).

  31. Adams, M. H., Bacteriophages (Interscience, New York, 1959).

  32. Shah, D. B., and Berger, H., J. molec. Biol., 57, 17–34 (1971).

    Article  CAS  PubMed  Google Scholar 

  33. Studier, F. W., J. molec. Biol., 11, 373–390 (1965).

    Article  CAS  PubMed  Google Scholar 

  34. Malamy, M. H., Fiandt, M., Szybalski, W., Molec. gen. Genet., 117, 207–222 (1972).

    Article  Google Scholar 

  35. MacKay, V., and Linn, S., J. biol. Chem., 249, 4286–4294 (1974).

    CAS  PubMed  Google Scholar 

  36. Revel, H. R., Virology, 31, 688–701 (1964).

    Article  Google Scholar 

  37. Fleischman, R. A., and Richardson, C. C., Proc. natn. Acad. Sci. U.S.A., 68, 2527–2531 (1971).

    Article  ADS  CAS  Google Scholar 

  38. Hewlett, M. J., and Mathews, C. K., J. Virol., 15, 776–784 (1975).

    CAS  PubMed  PubMed Central  Google Scholar 

  39. Altman, S., and Meselson, M., Proc. natn. Acad. Sci. U.S.A., 66, 716–721 (1970).

    Article  ADS  CAS  Google Scholar 

  40. Tanner, D., and Oishi, M., Biochim. biophys. Acta, 228, 764–769 (1971).

    Article  Google Scholar 

  41. Sakaki, Y., J. Virol., 15, 1611–1612 (1974).

    Google Scholar 

  42. Dharmalingam, K., and Goldberg, E. B., Nature, 260, 000–000 (1976).

    Google Scholar 

  43. Benzinger, R., and McCorquodale, D. J., J. Virol., 16, 1–4 (1975).

    CAS  PubMed  PubMed Central  Google Scholar 

  44. Studier, F. W., J. molec. Biol., 94, 283–295 (1975).

    Article  CAS  PubMed  Google Scholar 

  45. Kornberg, A., DNA Synthesis. (Freeman, San Francisco, 1974).

    Google Scholar 

  46. Luria, S. E., and Human, M. L., J. Bact., 64, 557–569 (1952).

    CAS  PubMed  PubMed Central  Google Scholar 

  47. Bertani, G., and Weigle, J. J., J. Bact., 65, 113–121 (1953).

    CAS  PubMed  PubMed Central  Google Scholar 

  48. Revel, H. R., and Luria, S. E., A. Rev. Genet., 4, 177–192 (1970).

    Article  CAS  Google Scholar 

  49. Terzi, M., J. molec. Biol., 34, 165–173 (1968).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, Massachusetts, 02111

    K. Dharmalingam & Edward B. Goldberg

Authors
  1. K. Dharmalingam
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Edward B. Goldberg
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dharmalingam, K., Goldberg, E. Mechanism localisation and control of restriction cleavage of phage T4 and λ chromosomes in vivo. Nature 260, 406–410 (1976). https://doi.org/10.1038/260406a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/260406a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing