Abstract
DNA copies of rabbit globin mRNA have been inserted into the plasmid mini-ColE1. After addition of poly(dT) tails, the plasmid was used to prime the copying of mRNA by reverse transcriptase. Mini-ColE1 carrying globin sequences were denatured and annealed with mini-ColE1 tailed with poly(dA). Functional plasmids containing the synthesised globin sequences were isolated by transfection in E. coli.
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Rabbitts, T. Bacterial cloning of plasmids carrying copies of rabbit globin messenger RNA. Nature 260, 221–225 (1976). https://doi.org/10.1038/260221a0
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DOI: https://doi.org/10.1038/260221a0
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