Abstract
REINITIATION of the growth of quiescent, non-transformed fibroblasts in tissue culture is achieved by addition of serum1,2 or low concentrations of certain polypeptide hormones3–6. These agents which are thought to interact primarily with the surface of the cell4 presumably require a second signal to be transmitted to the interior. An increase in the intracellular “availability” of low molecular weight nutrients has been suggested to fulfil such a role7,8. Mouse fibroblasts placed in different media depleted in certain low molecular weight nutrients reversibly ceased growth in either the G1/G0 phase (phosphate, histidine plus glutamine deprivation) or at random (methionine, leucine) in the cell cycle9,10. We have found that insulin and other agents stimulated these cells (except those arrested by leucine depletion) to synthesise DNA and divide, whereas fibroblast growth factor (FGF) which normally initiates cell growth in quiescent cultures arrested predominantly by serum depletion of the medium5,6 was ineffective. This suggests a possible differential basis for their normal mode of action.
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KAMELY, D., RUDLAND, P. Nutrient-dependent arrest of fibroblast growth is partially reversed by insulin but not fibroblast growth factor. Nature 260, 51–53 (1976). https://doi.org/10.1038/260051a0
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DOI: https://doi.org/10.1038/260051a0
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