Abstract
LOOSE connective tissue fibroblasts in the mouse become markedly non-motile and refractile and lose pinocytotic activity within about an hour of hydrocortisone infusion. Only minutes are required to mimic this response in culture1. These responses of fibroblasts are well correlated with the topical anti-inflammatory potency of the glucocorticoids2. Glucocorticoids inhibit the multiplication in primary cultures of rat and mouse fibroblasts and of human epithelial cells3–6. In marked contrast, they stimulate multiplication of 3T3 cells7. Some investigators regard the 3T3 cell as a prototype for the study of growth regulation in cultured fibroblasts, although this line has been identified as tumorigenic in some conditions8.
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FODGE, D., RUBIN, H. Differential effects of glucocorticoids on DNA synthesis in normal and virus-transformed chick embryo cells. Nature 257, 804–806 (1975). https://doi.org/10.1038/257804a0
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DOI: https://doi.org/10.1038/257804a0
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