Abstract
ULTRAVIOLET light-induced incorporation of 3H-thymidine into DNA by non-S-phase nuclei has been observed in cultured mammalian cells1, spermatogenic cells2–4, and embryos5. This unscheduled DNA synthesis is generally considered to be the result of excision and replacement of ultraviolet-damaged bases by repair enzymes6–8. We have examined the repair capacity of full-grown mouse oocytes after exposure to ultraviolet irradiation. As sensitivity to chemical mutagens and X irradiation changes during meiotic maturation in many species, we determined whether the ability to repair damage to DNA changes during mouse oocyte maturation in vitro. Oocytes were found to be capable of unscheduled DNA synthesis during meiotic maturation; however, irradiation of germinal vesicle stage oocytes induced higher levels of 3H-thymidine incorporation than irradiation at metaphase I or metaphase II stages.
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References
Rasmussen, R. E., and Painter, R. B., Nature, 203, 1360–1362 (1964).
Kofman-Alfaro, S., and Chandley, A. C., Expl Cell Res., 69, 33–44 (1971).
Chandley, A. C., and Kofman-Alfaro, S., Expl Cell Res., 69, 45–48 (1971).
Gledhill, B. L., and Darzynkiewicz, Z., J. exp. Zool., 183, 375–382 (1973).
Pedersen, R. A., and Cleaver, J. E., Expl Cell Res. (in the press)
Painter, R. E., and Cleaver, J. E., Radiat. Res., 37, 451–466 (1969).
Cleaver, J. E., Adv. Radiat. Biol., 4, 1–75 (1974).
Setlow, R. B., Prog. Nucl. Acid Res. molec. Biol., 8, 257–295 (1968).
Biggers, J. D., Whittingham, D. G., and Donahue, R. P., Proc. natn. Acad. Sci. U.S.A., 58, 560–567 (1967).
Sega, G. A., Proc. natn. Acad. Sci. U.S.A., 71, 4955–4959 (1974).
Cleaver, J. E., Cancer Res., 33, 362–369 (1973).
Russell, W. L., Russell, L. B., and Kelly, E. M., Science, 128, 1546–1550 (1958).
Russell, W. L., in Recovery and Repair Mechanisms in Radiology (Brookhaven Symp. Biol., 20, 179–187, 1968).
Russell, L. B., and Saylors, C. L., Repair from Genetic Radiation Damage and Differential Radiosensitivity in Germ Cells (edit. by Sobels, F.H. ), 313–340 (Macmillan, New York, 1963).
Masui, Y., J. embryol. exp. Morph., 29, 87–104; 29, 105–116 (1973).
Baker, T. G., Mutat. Res., 11, 9–22 (1971).
Edwards, R. G., and Searle, A. G., Genet. Res., 4, 389–398 (1963).
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MASUI, Y., PEDERSEN, R. Ultraviolet light-induced unscheduled DNA synthesis in mouse oocytes during meiotic maturation. Nature 257, 705–706 (1975). https://doi.org/10.1038/257705a0
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DOI: https://doi.org/10.1038/257705a0
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