Abstract
SCRAPIE, a naturally occurring slow virus disease of sheep and goats, has been transmitted experimentally to the mouse, rat, gerbil, mink, hamster, vole and monkey1. The agent has shown an unusual resistance to ultraviolet irradiation2, nucleases3 and β-propiolactone4. The precise nature of the scrapie agent, however, has yet to be defined. It is not known whether it is a virus, some type of self-replicating cell membrane, or part of a membrane5. The viroid hypothesis of the scrapie agent of Diener6 has been discounted7,8. Several different particles have been seen in thin sections of naturally and experimentally infected scrapie tissues9–11. As repeated fluorocarbon extractions of mink tissues infected with Aleutian disease virus (ADV), which causes persistent infection of mink, have resulted in isolation of the virus12,13, we thought it worthwhile to apply the same physico-chemical techniques in an attempt to isolate virus from mouse brains infected with scrapie agent.
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CHO, H., GREIG, A. Isolation of 14-nm virus-like particles from mouse brain infected with scrapie agent. Nature 257, 685–686 (1975). https://doi.org/10.1038/257685a0
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DOI: https://doi.org/10.1038/257685a0
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