Abstract
POLYAMINE biosynthesis is one of the earliest events occurring during tissue growth, and the polyamines have been implicated in the control of both RNA and protein synthesis. Increased polyamine synthesis reflects a rapid elevation of the activity of ornithine decarboxylase (ODC), the rate-limiting enzyme in the biosynthesis of the polyamines spermidine and spermine1,2. Fast growing tissues exhibit the highest ODC activity, whereas in slowly growing or in non-growing tissues ODC activity is very low. ODC has been induced by various agents that affect growth processes, such as by glucocorticoids in vivo3,6, and by cyclic AMP both in vivo6,7 and in cultured baby hamster kidney cells8. There have also been conflicting reports about the efficacy of polyamines in inhibiting the induction of ODC in vivo9–11 and in activated human lymphocytes12. As most results reported so far are from whole animal studies, we felt that a more precise understanding of ODC induction may result from investigation of an isolated system. The system chosen for detailed study was rat hepatoma cells (HTC) because most in vivo studies have focused on ODC from rat liver. We now report the induction of ODC in HTC cells by both cyclic AMP and dexamethasone.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Pegg, A. E., and Williams-Ashman, H. G., Biochem. J., 108, 533–539 (1968).
Williams-Ashman, H. G., Pegg, A. E., and Lockwood, D. H., Adv. Enzyme Regul., 7, 291–323 (1969).
Richman, R. A., Underwood, L. E., Van Wyk, J. J., and Voina, S. J., Proc. Soc. exp. Biol. Med., 138, 880–884 (1971).
Rizzo, A. J., Heilpern, P., and Webb, T. E., Cancer Res., 31, 876–881 (1971).
Cavia, E., and Webb, T. E., Biochim. Biophys. Acta, 262, 546–554 (1972).
Beck, W. Y., Bellantone, R. A., and Canellakis, E. S., Biochem. Biophys. Res. Commun., 48, 1649–1655 (1972).
Hölttä, E., and Raina, A., Acta endocr., Copenh., 73, 794–800 (1973).
Hogan, B., Shields, R., and Curtis, D., Cell, 2, 229–233 (1974).
Schrock, T. R., Oakman, N. J., and Bucher, N. L. R., Biochim. Biophys. Acta, 204, 564–577 (1970).
Jänne, J., and Williams-Ashman, H. G., J. Biol. Chem., 246, 1725–1732 (1971).
Jänne, J., and Hölttä, E., Biochem. Biophys. Res. Commun., 61, 449–456 (1974).
Kay, J. E., and Lindsay, V. J., Biochem. J., 132, 791–796 (1973).
Theoharides, T. C., Rec. Progr. Hormone Res. (in the press).
Gelehrter, T., Emanuel, J. R., and Spencer, C. J., J. Biol. Chem., 247, 6197–6203 (1972).
Russell, D. H., and Snyder, S. H., Proc. natn. Acad. Sci. U.S.A., 60, 1420–1427 (1968).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
THEOHARIDES, T., CANELLAKIS, Z. Spermine inhibits induction of ornithine decarboxylase by cyclic AMP but not by dexamethasone in rat hepatoma cells. Nature 255, 733–734 (1975). https://doi.org/10.1038/255733a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/255733a0
This article is cited by
-
Growth of exocrine acinar cells on a reconstituted basement membrane gel
In Vitro Cellular & Developmental Biology (1987)
-
The rapid transient stimulation of both cytoplasmic and mitochondrial ornithine decarboxylases in the liver of thyroidectomized rats by 3,5,3′-triiodo-L-thyronine
Bioscience Reports (1981)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.