Abstract
THE complexity of the central nervous system of vertebrates makes it difficult to identify the cell type responsible for the biochemical or pharmacological effects of drugs observed in the whole tissue. This difficulty does not exist for clonal cell lines derived from tumours of the nervous system. Such lines derived from mouse neuroblastoma C1300 show a number of differentiated functions of neurones1. Many of these properties are more strongly expressed in clonal lines of hybrid cells obtained by fusion of mouse neuroblastoma and rat glioma cells (refs 2–4 and B.H., unpublished). In suitable conditions, these undifferentiated cells differentiate by extending long processes3–5, which contain structures resembling synaptic vesicles6. In addition, their membranes become excitable by an electric current (refs 3 and 4 and B.H., unpublished) or by neurotransmitters (refs 3, 4 and 7, and B.H., unpublished). On exposure to prostaglandin E1 (PGE1) hybrid cells strongly increase their intracellular levels of cyclic AMP8. This effect of PGE1 is antagonised by α adrenergic agonists7 and morphine7,9.
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TRABER, J., FISCHER, K., BUCHEN, C. et al. Muscarinic response to acetylcholine in neuroblastoma × glioma hybrid cells. Nature 255, 558–560 (1975). https://doi.org/10.1038/255558a0
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DOI: https://doi.org/10.1038/255558a0
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