Abstract
REPLICATION in eukaryotic cells includes not only DNA synthetic processes1–4 but also the temporally coordinate5–7 and non-coordinate8,9,21 synthesis of chromatin proteins as well as assembly of these constituents into interphase chromosomes. While much is known about DNA histone and non-histone chromatin protein biosynthesis less is known about the time place and manner of association of proteins with DNA. This communication presents evidence that (1) DNA is replicated with pre-existent chromatin protein still attached; (2) chromatin proteins protect DNA from nuclease digestion in a characteristic manner unchanged by replicative processes and (3) addition of new chromatin protein to nascent DNA occurs after a measurable lag period.
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SEALE, R. Assembly of DNA and protein during replication in HeLa cells. Nature 255, 247–249 (1975). https://doi.org/10.1038/255247a0
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DOI: https://doi.org/10.1038/255247a0
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