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In vitro cultivation of Trypanosoma vivax isolated from cattle

Nature volume 251, pages 513–514 (1974)Cite this article

Abstract

IN West Africa, Trypanosoma vivax is responsible for the majority of trypanosome infections in cattle and other ruminants. It is not readily established in laboratory animals, and research on pathogenic isolates has been hampered by the difficulty in its establishment in vitro. Trager1 reported a culture method for T. vivax using tsetse fly tissues but this medium is complex and unsuitable for metabolic studies. There is therefore a need for a medium which will support the in vitro growth of T. vivax and other species of trypanosomes to give reproducible results. The multiplication of T. vivax, using mammalian tissue culture media buffered with 4-(2-hydroxy-ethyl)-1-piperazime-ethane-sulphonic acid (HEPES) is reported here.

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References

  1. Trager, W., Nature, 184, 30 (1959).

    Article  Google Scholar 

  2. Lanham, S. M., and Godfrey, D. G., Expl Parasitol., 28, 521 (1970).

    Article  CAS  Google Scholar 

  3. Leeflang, P., Blotkamp, C., Godfrey, D. G., and Kilgour, V., Trans R. Soc. trop. Med. Hyg. (in the press).

  4. Vickerman, K., Nature, 208, 762 (1965).

    Article  ADS  CAS  Google Scholar 

  5. Splitter, E. J., and Soulsby, E. J. L., Expl Parasitol., 21, 137 (1967).

    Article  CAS  Google Scholar 

  6. Pittam, M. D., Comp. Biochem. Physiol., 33, 127 (1970).

    Google Scholar 

  7. Hoare, C. A., The Trypanosomes of Mammals (Blackwell Scientific Publications, Oxford).

  8. Good, N. E., Winget, G. D., Winter, W., Connolly, T. N., Izawa, S., and Singh ., Biochemistry, 5, 466 (1966).

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Veterinary Pathology, Department of Veterinary Anatomy and Physiology, University of Ibadan, Nigeria

    T. T. ISOUN & MIRIAM J. ISOUN

Authors
  1. T. T. ISOUN
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  2. MIRIAM J. ISOUN
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ISOUN, T., ISOUN, M. In vitro cultivation of Trypanosoma vivax isolated from cattle. Nature 251, 513–514 (1974). https://doi.org/10.1038/251513a0

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  • DOI: https://doi.org/10.1038/251513a0

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